Cappable-seq is a method for directly enriching the 5´ end of primary transcripts developed at NEB. This is achieved by capping the 5´ triphosphorylated end of RNA with the Vaccinia Capping System (NEB #M2080) and 3´-Desthiobiotin-GTP (NEB #N0761). The primary transcripts are enriched by binding to Hydrophilic Streptavidin Magnetic Beads (NEB #S1421), followed by washing and eluting with biotin.The method enables determination of transcription start sites at single base resolution (1).
- Ettwiller, L. et al. (2016). BMC Genomics. 17, 199.
- Wulf, Madalee; Buswell, John; Chan, Siuhong; Dai, Nan; Marks, Katherine; Tzertzinis, George; Whipple, Joe; Correa, Ivan; Schildkraut, Ira; 2019. The yeast scavenger decapping enzyme DcpS and its application for in vitro RNA recapping Sci Rep. 9 (1), PubMedID: 31197197, DOI: 10.1038/s41598-019-45083-5
This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).
While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
For more information about commercial rights, please contact NEB's Global Business Development team at email@example.com.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.