Cappable-seq

Cappable-seq is a method for directly enriching the 5´ end of primary transcripts developed at NEB. This is achieved by capping the 5´ triphosphorylated end of RNA with the Vaccinia Capping System (NEB #M2080) and 3´-Desthiobiotin-GTP (NEB #N0761). The primary transcripts are enriched by binding to Hydrophilic Streptavidin Magnetic Beads (NEB #S1421), followed by washing and eluting with biotin.The method enables determination of transcription start sites at single base resolution (1).

 

References

  1. Ettwiller, L. et al. (2016). BMC Genomics. 17, 199.
 

Choose Type:

Protocols for Cappable-seq

    Other Tools & Resources

    Brochures

  • Latest NEB Expressions

    This edition of NEB expressions discusses breakthroughs in Golden Gate Assembly research, that now enables 20+ fragment assembly with high efficiency and accuracy. New products include the NEBNext® Single Cell/Low Input RNA Library Prep Kit and the NEBNext Direct® Custom Ready Panels for NGS Target Enrichment. Lastly, learn about the importance of basic research at NEB in an interview with NEB’s Research Director.

  • RNA Technical Guide

    Find in depth information on tools designed to streamline your RNA workflows, including RNA extraction & purification, qPCR and RT-qPCR, RNA-seq, RNA synthesis and gRNA synthesis.