Cappable-seq is a method for directly enriching the 5´ end of primary transcripts developed at NEB. This is achieved by capping the 5´ triphosphorylated end of RNA with the Vaccinia Capping System (NEB #M2080) and 3´-Desthiobiotin-GTP (NEB #N0761). The primary transcripts are enriched by binding to Hydrophilic Streptavidin Magnetic Beads (NEB #S1421), followed by washing and eluting with biotin.The method enables determination of transcription start sites at single base resolution (1).



  1. Ettwiller, L. et al. (2016). BMC Genomics. 17, 199.

Protocols for Cappable-seq

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