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Ion Torrent® DNA Library Preparation

In general, library preparation of fragmented DNA, for use with the Ion Torrent™ sequencing platform, involves repair of 3′ and 5′ ends before ligation to an adaptor. After ligation, adaptor-DNA constructs are purified and size selected, and amplified via polymerase chain reaction (PCR). For more information about DNA Library Construction, choose the workflow tabs below.

Libraries prepared in this manner are suitable for template preparation, the next step in the Ion Torrent sequencing workflow.

NEBNext® reagents are available for each step in the DNA library preparation workflow for the Ion Torrent platform. For DNA library preparation, reagents from NEB are available with or without reagents for enzyme-based fragmentation. Each of these reagents has been functionally validated by preparation of a genomic DNA library followed by Ion Torrent sequencing. For representative results of both library construction using NEBNext and Ion Torrent run results, choose the tabs below.

Ion Torrent® is a trademark owned byThermo Fischer Scientific.

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Ion Torrent® DNA Library Preparation includes these areas of focus:
DNA for Ion Torrent®
Protocols for Ion Torrent® DNA Library Preparation
DNA Preparation Workflow for Ion Torrent
Representative Results from Libraries Constructed Using NEBNext for Ion Torrent
Representative Ion Torrent Run Report
Legal Information
This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

Videos

  1. NEBNextVideo_WhichKit_thumb

    Which DNA Library Prep Kit for Ion Torrent Should I Choose?

    Having trouble deciding which NEBNext Fast DNA Library Prep Set for Ion Torrent™ to choose? Let Christine explain which set is right for your DNA.

  2. BeckmanAutomation_ProductivityWebinar_thumb_0715

    Increase Productivity & Reproducibility by Automating DNA Library Prep for Ion Torrent™ Sequencing

    Learn how Biomek automation of NEBNext DNA library preparation for Ion Torrent enables high-throughput, reproducible production of libraries with high yield and complexity. In this webinar Joanna Hamilton from Dartmouth Genomics & Molecular Biology Shared Resource, Lynne Apone from New England Biolabs and Zach Smith from Beckman Coulter describe the NEBNext Fast Fragmentation & DNA Library Prep kit for Ion Torrent, automation of this method and implementation in multiple workflows in a sequencing core lab.

  3. NEBNextVideo_OptimizingDNAInput_thumb

    Tips for Optimizing DNA Inputs in NGS Library Construction

    Optimizing DNA inputs for NGS library prep is an important step, if you want to ensure a high-quality library. Start by following these tips for DNA quantification and assessing purity.

  4. NEBNextVideo_BeadBasedCleanup_thumb

    Tips for Bead-based Clean ups and Size Selection

    Using beads to clean up your DNA prior to NGS library prep can be quick and easy, if you follow these few, simple tips.

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