Protocols for Fast PCR
- Loop-mediated Isothermal Amplification (LAMP)
- PCR Optimization (E0555)
- PCR Optimization of the Control Template using Phusion® High-Fidelity PCR Kit
- PCR Optimization with Phusion® High-Fidelity PCR Kit
- PCR Protocol for Phusion High-Fidelity DNA Polymerase (M0530)
- PCR Protocol for Phusion® Hot Start Flex DNA Polymerase (M0535)
- PCR Using NEBNext® High-Fidelity 2X PCR Master Mix (M0541)
- PCR Using Q5® High-Fidelity DNA Polymerase (M0491)
- PCR Using Q5® Hot Start High-Fidelity DNA Polymerase (M0493)
- Protocol for a PCR reaction using NEBNext® Q5® Hot Start HiFi PCR Master Mix (M0543)
- Protocol for a Routine PCR (E0555)
- Protocol for a Routine PCR with Phusion® High-Fidelity PCR Kit
- Protocol for OneTaq 2X Master Mix with GC Buffer (M0483)
- Protocol for OneTaq Hot Start 2X Master Mix with Standard Buffer (M0484)
- Protocol for OneTaq® 2X Master Mix with Standard Buffer (M0482)
- Protocol for Phusion® High-Fidelity PCR Master Mix with GC Buffer
- Protocol for Phusion® Hot Start Flex 2X Master Mix
- Protocol for Q5® High-Fidelity 2X Master Mix
- Protocol for Q5® Hot Start High-Fidelity 2X Master Mix
- Protocol Phusion® High-Fidelity PCR Master Mix with HF Buffer
Anatomy of a Polymerase - How Structure Effects Function
Polymerase Fidelity: What is it, and what does it mean for your PCR?
Understanding Variability in DNA Amplification Reactions
- DNA Polymerase Selection Chart
- PCR Troubleshooting Guide
- Taq PCR Kit Troubleshooting Guide
- General Guidelines for Successful RNA Purification Using the Monarch Total RNA Miniprep Kit
- Guidance on Choosing Sample Input Amounts when Using the Monarch Total RNA Miniprep Kit
- Guidelines for PCR Optimization with OneTaq® and OneTaq® Hot Start DNA Polymerases
- Guidelines for PCR Optimization with Taq DNA Polymerase
- Guidelines for PCR Optimization with Thermophilic DNA Polymerases
Other Tools & Resources
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For more information about commercial rights, please contact NEB's Global Business Development team at firstname.lastname@example.org.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
NEB offers a variety of DNA polymerases for all of your experimental needs. High-fidelity, multiplex, and fast PCR are just a few of the types of amplification reactions you may need to conduct, and Breton explains which polymerases to choose.