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  • Glycobiology Unit Conversion Chart

    Reagent companies differ in how a unit of enzyme is defined. This chart can be used to help determine how a unit of enzyme from one company compares to a unit of enzyme from NEB. All enzymes were assayed using NEB's assay protocols as a means of normalization (NEB Assay).

    Enzyme Company Selling Conc. (U/ml) Units /Vial µl/Vial NEB Assay (U/ml) NEB Assay Units /Vial µl Conversion (1 NEB µl = x Company µls)
    PNGase F NEB (NEB #P0704/P0705) 500,000 15,000 30 500,000 15,000 1
    Prozyme (GKE-5006A) 2.5 0.1 40 150,000 6,000 3.3
    Prozyme (GKE-5020B, Ultra) 10 0.4 40 500,000 20,000 1
    QA Bio (E-PNG01) 5 0.3 60 200,000 12,000 2.5
    Sigma (P7367) 500 50 50 90,000 4,500 5.5
    Endo H NEB (NEB #P0702) 500,000 10,000 20 500,000 10,000 1
    Prozyme (GKE-5002) 0.15 0.03 200 30,000 6,000 16.5
    α1-6 Mannosidase NEB (NEB #P0727) 40,000 800 20 40,000 800 1
    Prozyme (GKX-80070) 0.2 .008 40 1,000 40 40
    Prozyme (GKX 5010, α1-2,3,6 Man) 150 10 66 4,000 264 10
    QA Bio (E-AMO1, α1-2,3,6 Man) 10 0.6 60 2,000 120 20
    α1-2,3 Mannosidase NEB (NEB #P0729) 32,000 640 20 32,000 640 1
    Prozyme (GKX 5010, α1-2,3,6 Man) 150 10 66 4,000 264 8
    QA Bio (E-AMO1, α1-2,3,6 Man) 10 0.6 60 700 42 45
    β1-4 Galactosidase NEB (NEB #P0730) 8,000 400 50 8,000 400 1
    Prozyme (GKX-5014) 2 0.2 100 8,000 800 1
    Prozyme (GKX 5013, β1-3,4 Gal) 5 0.5 100 2,000 200 4
    QA Bio (E-BG07) 3 0.18 60 40,000 2,400 0.2
    QA Bio (E-BG02, β1-3,4,6 Gal) 2.5 0.5 200 4,000 800 2
    β1-3 Galactosidase NEB (NEB #P0726) 10,000 500 50 10,000 500 1
    Prozyme (GKX 5013, β1-3,4 Gal) 5 0.5 100 10,000 1000 1
    QA Bio (E-BG02, β1-3,4,6 Gal) 2.5 0.5 200 2,000 400 5
    β-N-Acetyl glucosaminidase NEB (NEB #P0732)* 4,000 100 25 4,000 100 1
    Prozyme (GK80050 40 1.6 40 2,000 80 2
    QA Bio (E-GL01) 40 2.4 60 2,000 120 2
    α-N-Acetyl galactosaminidase NEB (NEB #P0734) 20,000 3,000 150 20,000 3,000 1
    Prozyme (GKX-5001) 5 0.1 20 600 12 33
    α1-3,6 Galactosidase NEB (NEB #P0731) 4,000 100 25 4,000 100 1
    Prozyme (GKX-5007, α1-3,4,6 Gal) 100 5 50 120,000 6,000 0.03
    Neuraminidase NEB (NEB #P0720) 50,000 2,000 40 50,000 2,000 1
    Prozyme (GKX-5021), α2-3, 6, 8 Neur) 5 1 200 10,000 2,000 5
    Prozyme (GK80040, α2-3,6,8,9 Neur) 5 1 200 5,000 1,000 10
    α2-3 Neuraminidase NEB (NEB #P0728) 5,000 2,500 50 50,000 2,500 1
    Prozyme (GK80020) 20 1 50 80,000 4,000 0.625
    β-N-Acetyl hexosaminidasef NEB (NEB #P0721) 5,000 500 100 5,000 500 1
    Prozyme (GKX-5003) 50 5 100 500 50 10
    α1-2 Fucosidase NEB (NEB #P0724) 20,000 1,000 50 20,000 1000 1
    Prozyme (GK80170) 0.2 0.004 20 500 10 40

    *NEB (P0732) requires a longer incubation time for cleavage of β1-2 linkages.