Home Resources Selection Charts Recommended HiScribe® RNA Synthesis Kits by Application

Recommended HiScribe® RNA Synthesis Kits by Application

  T7 KITS SP6 KITS

APPLICATION HISCRIBE T7 HIGH YIELD RNA SYNTHESIS KIT (#E2040) HISCRIBE T7 QUICK HIGH YIELD RNA SYNTHESIS KIT (#E2050) HISCRIBE T7 ARCA mRNA KIT (#E2065) HISCRIBE T7 ARCA mRNA KIT (WITH TAILING) (#E2060) HISCRIBE T7 mRNA KIT WITH CLEANCAP®  REAGENT AG  (#E2080) HISCRIBE SP6 RNA SYNTHESIS KIT (#E2070)
PROBE LABELING

Fluorescent labeling: FAM, Cyanine (Cy) dyes, etc.
• Fluorescent in situ hybridization (FISH)

 ✓ 
      ✓

Non-fluorescent labeling: Biotin, Digoxigenin
• In situ hybridization
• Blot hybridization with secondary detection
• Microarray

   ✓       ✓
High specific activity radiolabeling
• Blot hybridization
• RNase protection		 ✓         ✓
mRNA & RNA FOR TRANSFECTION Streamlined high yield CleanCap Reagent AG capped RNA synthesis
• Template encoded poly(A) tails
• Non polyadenylated transcripts
• Transfection
• Microinjection
• In vitro translation		         ✓
Streamlined mRNA synthesis with ARCA co-transcriptional capping and enzymatic poly(A) tailing
• Transfection
• Microinjection
• In vitro translation		       ✓  
Streamlined ARCA capped RNA synthesis
• Template encoded poly(A) tails
• Non polyadenylated transcripts
• Transfection
• Microinjection
• In vitro translation		     ✓    
Co-transcriptional capping with alternate cap analogs
• Transfection
• Microinjection
• In vitro translation		 ✓ ✓       ✓
Post-transcriptional capping with Vaccinia mRNA Capping System
• Transfection
• Microinjection
• In vitro translation		 ✓ ✓       ✓
Complete substitution of NTPs: 5-mC, pseudouridine, etc.
• Post-transcriptional capping with Vaccinia mRNA Capping System		 ✓       ✓ ✓
Partial substitution of NTPs: 5-mC, pseudouridine, etc.   ✓ ✓ ✓ ✓ ✓
Unmodified RNA   ✓     ✓ ✓
Hairpins, short RNA, dsRNA
• Gene knockdown		   ✓       ✓
STRUCTURE, FUNCTION, & BINDING STUDIES Complete substitution of NTPs • Aptamer selection
• Isotopic labeling		 ✓         ✓
Partial substitution of one or more NTPs
• Aptamer selection
• Structure determination		   ✓       ✓
Unmodified RNA
• SELEX
• Structure determination		   ✓       ✓