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Home Tools & Resources Selection Charts Recommended HiScribe RNA Synthesis Kits by Application

Recommended HiScribe RNA Synthesis Kits by Application

		T7 KITS				SP6 KITS
	APPLICATION	HISCRIBE T7 HIGH YIELD RNA SYNTHESIS KIT (#E2040)	HISCRIBE T7 QUICK HIGH YIELD RNA SYNTHESIS KIT (#E2050)	HISCRIBE T7 ARCA MRNA KIT (#E2065)	HISCRIBE T7 ARCA MRNA KIT (WITH TAILING) (#E2060)	HISCRIBE SP6 RNA SYNTHESIS KIT (#E2070)
PROBE LABELING	Fluorescent labeling: FAM, Cyanine (Cy) dyes, etc. • Fluorescent in situ hybridization (FISH)		✓			✓
	Non-fluorescent labeling: Biotin, Digoxigenin • In situ hybridization • Blot hybridization with secondary detection • Microarray		✓			✓
	High specific activity radiolabeling • Blot hybridization • RNase protection	✓				✓
mRNA & RNA FOR TRANSFECTION	Streamlined mRNA synthesis with ARCA co-transcriptional capping and enzymatic poly(A) tailing • Transfection • Microinjection • In vitro translation				✓
	Streamlined ARCA capped RNA synthesis • Template encoded poly(A) tails • Non polyadenylated transcripts • Transfection • Microinjection • In vitro translation			✓
	Co-transcriptional capping with alternate cap analogs • Transfection • Microinjection • In vitro translation		✓			✓
	Post-transcriptional capping with Vaccinia mRNA Capping System • Transfection • Microinjection • In vitro translation	✓	✓			✓
	Complete substitution of NTPs: 5-mC, pseudouridine, etc. • Post-transcriptional capping with Vaccinia mRNA Capping System	✓				✓
	Partial substitution of NTPs: 5-mC, pseudouridine, etc.		✓	✓	✓	✓
	Unmodified RNA		✓			✓
	Hairpins, short RNA, dsRNA • Gene knockdown		✓			✓
STRUCTURE, FUNCTION, & BINDING STUDIES	Complete substitution of NTPs • Aptamer selection • Isotopic labeling	✓				✓
	Partial substitution of one or more NTPs • Aptamer selection • Structure determination					✓
	Unmodified RNA • SELEX • Structure determination		✓			✓

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