Recommended HiScribe RNA Synthesis Kits by Application
| |
T7 KITS |
SP6 KITS |
||||
|---|---|---|---|---|---|---|
| APPLICATION | HISCRIBE T7 HIGH YIELD RNA SYNTHESIS KIT (#E2040) |
HISCRIBE T7 QUICK HIGH YIELD RNA SYNTHESIS KIT (#E2050) |
HISCRIBE T7 ARCA MRNA KIT (#E2065) |
HISCRIBE T7 ARCA MRNA KIT (WITH TAILING) (#E2060) |
HISCRIBE SP6 RNA SYNTHESIS KIT (#E2070) |
|
| PROBE LABELING |
Fluorescent labeling: FAM, Cyanine (Cy) dyes, etc. |
✓ | ✓ | |||
|
Non-fluorescent labeling: Biotin, Digoxigenin |
✓ | ✓ | ||||
| High specific activity radiolabeling • Blot hybridization • RNase protection |
✓ | ✓ | ||||
| mRNA & RNA FOR TRANSFECTION |
Streamlined mRNA synthesis with ARCA co-transcriptional capping and enzymatic poly(A) tailing • Transfection • Microinjection • In vitro translation |
✓ | ✓ | |||
| Streamlined ARCA capped RNA synthesis • Template encoded poly(A) tails • Non polyadenylated transcripts • Transfection • Microinjection • In vitro translation |
✓ | ✓ | ||||
| Co-transcriptional capping with alternate cap analogs • Transfection • Microinjection • In vitro translation |
✓ | ✓ | ||||
| Post-transcriptional capping with Vaccinia mRNA Capping System • Transfection • Microinjection • In vitro translation |
✓ | ✓ | ✓ | |||
| Complete substitution of NTPs: 5-mC, pseudouridine, etc. • Induction of stem cell pluripotency • Modulation of cell fate or phenotype • Post translational capping with Vaccinia mRNA Capping System |
✓ | ✓ | ||||
| Partial substitution of NTPs: 5-mC, pseudouridine, etc. | ✓ | ✓ | ✓ | ✓ | ||
| Unmodified RNA | ✓ | ✓ | ||||
| Hairpins, short RNA, dsRNA • Gene knockdown |
✓ | ✓ | ||||
| STRUCTURE, FUNCTION, & BINDING STUDIES |
Complete substitution of NTPs • Aptamer selection • Isotopic labeling |
✓ | ✓ | |||
| Partial substitution of one or more NTPs • Aptamer selection • Structure determination |
✓ | |||||
| Unmodified RNA • SELEX • Structure determination |
✓ | ✓ | ||||
