Recommended HiScribe RNA Synthesis Kits by Application

 
T7 KITS
SP6 KITS

APPLICATION HISCRIBE
T7 HIGH
YIELD RNA
SYNTHESIS
KIT (#E2040)
HISCRIBE
T7 QUICK
HIGH YIELD
RNA
SYNTHESIS
KIT
(#E2050)
HISCRIBE
T7 ARCA
MRNA KIT
(#E2065)
HISCRIBE
T7 ARCA
MRNA KIT
(WITH TAILING)
(#E2060)
HISCRIBE
SP6 RNA
SYNTHESIS KIT
(#E2070)
PROBE LABELING

Fluorescent labeling: FAM, Cyanine (Cy) dyes, etc.
• Fluorescent in situ hybridization (FISH)

     

Non-fluorescent labeling: Biotin, Digoxigenin
In situ hybridization
• Blot hybridization with secondary detection
• Microarray

     
High specific activity radiolabeling
• Blot hybridization
• RNase protection
     
mRNA & RNA FOR
TRANSFECTION
Streamlined mRNA synthesis with ARCA co-transcriptional
capping and enzymatic poly(A) tailing
• Transfection
• Microinjection
In vitro translation
     
Streamlined ARCA capped RNA synthesis
• Template encoded poly(A) tails
• Non polyadenylated transcripts
• Transfection
• Microinjection
In vitro translation
     
Co-transcriptional capping with alternate cap analogs
• Transfection
• Microinjection
In vitro translation
     
Post-transcriptional capping with Vaccinia mRNA Capping System
• Transfection
• Microinjection
In vitro translation
   
Complete substitution of NTPs: 5-mC, pseudouridine, etc.
• Induction of stem cell pluripotency
• Modulation of cell fate or phenotype
• Post translational capping with Vaccinia mRNA Capping System
     
Partial substitution of NTPs: 5-mC, pseudouridine, etc.  
Unmodified RNA      
Hairpins, short RNA, dsRNA
• Gene knockdown
     
STRUCTURE,
FUNCTION,
& BINDING STUDIES
Complete substitution of NTPs
• Aptamer selection
• Isotopic labeling
     
Partial substitution of one or more NTPs
• Aptamer selection
• Structure determination
       
Unmodified RNA
• SELEX
• Structure determination