Recommended HiScribe RNA Synthesis Kits by Application
| T7 KITS | SP6 KITS | ||||||
|---|---|---|---|---|---|---|---|
| |
APPLICATION | HISCRIBE T7 HIGH YIELD RNA SYNTHESIS KIT (#E2040) | HISCRIBE T7 QUICK HIGH YIELD RNA SYNTHESIS KIT (#E2050) | HISCRIBE T7 ARCA mRNA KIT (#E2065) | HISCRIBE T7 ARCA mRNA KIT (WITH TAILING) (#E2060) | HISCRIBE T7 mRNA KIT WITH CLEANCAP® REAGENT AG (#E2080) | HISCRIBE SP6 RNA SYNTHESIS KIT (#E2070) |
| PROBE LABELING | Fluorescent labeling: FAM, Cyanine (Cy) dyes, etc. |
✓ | ✓ | ||||
Non-fluorescent labeling: Biotin, Digoxigenin |
✓ | ✓ | |||||
| High specific activity radiolabeling • Blot hybridization • RNase protection |
✓ | ✓ | |||||
| mRNA & RNA FOR TRANSFECTION | Streamlined high yield CleanCap Reagent AG capped RNA synthesis • Template encoded poly(A) tails • Non polyadenylated transcripts • Transfection • Microinjection • In vitro translation |
✓ | |||||
| Streamlined mRNA synthesis with ARCA co-transcriptional
capping and enzymatic poly(A) tailing • Transfection • Microinjection • In vitro translation |
✓ | ||||||
| Streamlined ARCA capped RNA synthesis • Template encoded poly(A) tails • Non polyadenylated transcripts • Transfection • Microinjection • In vitro translation |
✓ | ||||||
| Co-transcriptional capping with alternate cap analogs • Transfection • Microinjection • In vitro translation |
✓ | ✓ | ✓ | ||||
| Post-transcriptional capping with Vaccinia mRNA Capping System • Transfection • Microinjection • In vitro translation |
✓ | ✓ | ✓ | ||||
| Complete substitution of NTPs: 5-mC, pseudouridine, etc. • Post-transcriptional capping with Vaccinia mRNA Capping System |
✓ | ✓ | ✓ | ||||
| Partial substitution of NTPs: 5-mC, pseudouridine, etc. | ✓ | ✓ | ✓ | ✓ | ✓ | ||
| Unmodified RNA | ✓ | ✓ | ✓ | ||||
| Hairpins, short RNA, dsRNA • Gene knockdown |
✓ | ✓ | |||||
| STRUCTURE, FUNCTION, & BINDING STUDIES | Complete substitution of NTPs
• Aptamer selection • Isotopic labeling |
✓ | ✓ | ||||
| Partial substitution of one or more NTPs • Aptamer selection • Structure determination |
✓ | ✓ | |||||
| Unmodified RNA • SELEX • Structure determination |
✓ | ✓ | |||||
