Although NEB offers different phosphatases, we recommend Quick CIP (NEB #M0525), as it has high specific activity, a rapid protocol, does not require any additives, and can be heat inactivated.
|Recombinant Shrimp Alkaline Phosphatase(rSAP)
| Antarctic Phosphatase
|100% heat inactivation||2 minutes/80°C
||5 minutes/65°C||2 minutes/80°C|
|High specific activity||✓
|Works directly in NEB buffers||✓
|Quick Protocol (10 min)||✓