Interrupted Palindromes

All recognition sequences are written 5' to 3' using the single letter code nomeclature with the point of cleavage indicated by a "/". Numbers in parentheses indicate distance from the recognition sequence.

For example, GGTCTC(1/5) indicates cleavage at:
5'... GGTCTCN ...3'
3'... CCAGAGNNNNN/ ...5'

Enzyme Recognition Sequences
AgsI TTS/AA
Δ AhdI GACNNN/NNGTC
Δ AleI CACNN/NNGTG
X AlfI (10/12)GCANNNNNNTGC(12/10)
Δ AlwNI CAGNNN/CTG
X ApaBI GCANNNNN/TGC
Δ ApeKI G/CWGC
Δ AvaII G/GWCC
Δ BglI GCCNNNN/NGGC
BisI GC/NGC
Δ BlpI GC/TNAGC
BlsI GCN/GC
BmgT120I G/GNCC
BplI (8/13)GAGNNNNNCTC(13/8)
Δ BsaBI GATNN/NNATC
Δ BsaJI C/CNNGG
Δ BsaWI W/CCGGW
Δ BsiHKAI GWGCW/C
Δ BslI CCNNNNN/NNGG
Δ BstAPI GCANNNN/NTGC
Δ BstEII § G/GTNACC
Δ BstNI CC/WGG
Δ BstXI CCANNNNN/NTGG
Δ Bsu36I CC/TNAGG
Δ Cac8I GCN/NGC
X CjuI CAYNNNNNRTG
Δ DdeI C/TNAG
Δ DrdI GACNNNN/NNGTC
Δ EcoNI CCTNN/NNNAGG
Δ EcoO109I RG/GNCCY
FalI (8/13)AAGNNNNNCTT(13/8)
X HaeI WGG/CCW
Δ DraIII-HF CACNNN/GTG
Δ BstEII-HF G/GTNACC
Δ HinfI G/ANTC
Δ Hpy166II GTN/NAC
Δ Hpy188I TCN/GA
Δ Hpy188III TC/NNGA
Δ Hpy99I CGWCG/
Δ HpyCH4III ACN/GT
KflI GG/GWCCC
MaeIII /GTNAC
X MjaIV GTNNAC
Δ MslI CAYNN/NNRTG
Δ MwoI GCNNNNN/NNGC
Δ NciI CC/SGG
Δ NlaIV GGN/NCC
PasI CC/CWGGG
PcsI WCGNNNN/NNNCGW
Δ PflFI GACN/NNGTC
Δ PflMI CCANNNN/NTGG
PfoI T/CCNGGA
Δ PpuMI RG/GWCCY
Δ PshAI GACNN/NNGTC
Δ PspGI /CCWGG
Δ RsrII CG/GWCCG
Δ Sau96I G/GNCC
Δ ScrFI CC/NGG
SetI ASST/
Δ SexAI A/CCWGGT
Δ SfiI GGCCNNNN/NGGCC
X Sse8647I AG/GWCCT
Δ StyD4I /CCNGG
Δ StyI § C/CWWGG
Δ StyI-HF C/CWWGG
TatI W/GTACW
Δ TfiI G/AWTC
Δ TseI G/CWGC
Δ Tsp45I /GTSAC
Δ TspRI CASTGNN/
X TssI GAGNNNCTC
Δ Tth111I GACN/NNGTC
Δ XcmI CCANNNNN/NNNNTGG
Δ XmnI GAANN/NNTTC
Note:
Enzymes marked with a (Δ) are available from NEB.
Enzymes marked with an (X) are not currently commercially available.
§ A HF version of this enzyme is available.