Interrupted Palindromes

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  • All recognition sequences are written 5' to 3' using the single letter code nomeclature with the point of cleavage indicated by a "/". Numbers in parentheses indicate distance from the recognition sequence.

    For example, GGTCTC(1/5) indicates cleavage at:
    5'... GGTCTCN ...3'
    3'... CCAGAGNNNNN/ ...5'

    EnzymeRecognition Sequence
    ΔAhdIGACNNN/NNGTC
    AlfI(10/12)GCANNNNNNTGC(12/10)
    AgsITTS/AA
    ΔAleICACNN/NNGTG
    ΔAlwNICAGNNN/CTG
    XApaBIGCANNNNN/TGC
    ΔApeKIG/CWGC
    ΔAvaIIG/GWCC
    ΔBglIGCCNNNN/NGGC
    BisIGC/NGC
    ΔBlpIGC/TNAGC
    BlsIGCN/GC
    ΔBsaBIGATNN/NNATC
    ΔRsrIICG/GWCCG
    ΔBsaJIC/CNNGG
    ΔBsaWIW/CCGGW
    ΔBsiHKAIGWGCW/C
    ΔBslICCNNNNN/NNGG
    ΔBssKI/CCNGG
    ΔBstAPIGCANNNN/NTGC
    ΔBstEII  (1) (2)G/GTNACC
    ΔBstNICC/WGG
    ΔBstXICCANNNNN/NTGG
    ΔBsu36ICC/TNAGG
    XBthCIGCNG/C
    BplI(8/13)GAGNNNNNCTC(13/8)
    BmgT120IGG/NCC
    XCjuICAYNNNNNRTG
    ΔCac8IGCN/NGC
    ΔDdeIC/TNAG
    ΔDraIII (1)CACNNN/GTG
    ΔDrdIGACNNNN/NNGTC
    XEcoHI/CCSGG
    ΔEcoNICCTNN/NNNAGG
    ΔEcoO109IRG/GNCCY
    FalI(8/13)AAGNNNNNCTT(13/8)
    XFmuIGGNC/C
    XHaeIWGG/CCW
    ΔHinfIG/ANTC
    ΔHpy99ICGWCG/
    ΔHpy188ITCN/GA
    ΔHpy188IIITC/NNGA
    ΔHpy166IIGTN/NAC
    ΔHpyCH4IIIACN/GT
    KflIGG/GWCCC
    MaeIII/GTNAC
    XMjaIVGTNNAC
    ΔMslICAYNN/NNRTG
    ΔMwoIGCNNNNN/NNGC
    ΔNciICC/SGG
    ΔNlaIVGGN/NCC
    XPasICC/CWGGG
    PcsIWCGNNNN/NNNCGW
    ΔPflFIGACN/NNGTC
    PfoIT/CCNGGA
    ΔPflMICCANNNN/NTGG
    ΔPpuMIRG/GWCCY
    ΔPshAIGACNN/NNGTC
    ΔPspGI/CCWGG
    XPspO3IGGWC/C
    XPssIRGGNC/CY
    ΔSau96IG/GNCC
    ΔScrFICC/NGG
    SetIASST/
    ΔSexAIA/CCWGGT
    ΔSfiIGGCCNNNN/NGGCC
    XSse8647IAG/GWCCT
    ΔStyD4I/CCNGG
    ΔStyI (1)C/CWWGG
    ΔTfiIG/AWTC
    TatIW/GTACW
    ΔTseIG/CWGC
    ΔTsp45I/GTSAC
    ΔTspRINNCASTGNN/
    XTssIGAGNNNCTC
    ΔTth111IGACN/NNGTC
    XUnbI/GGNCC
    XVpak11AI/GGWCC
    ΔXcmICCANNNNN/NNNNTGG
    ΔXmnIGAANN/NNTTC
    Note:
    Enzymes marked with a (Δ) are available from NEB.
    Enzymes marked with an (X) are not currently commercially available.
    (1) A HF version of this enzyme is available.
    (2) A RE-Mix version of this enzyme is available.