Interrupted Palindromes

All recognition sequences are written 5' to 3' using the single letter code nomeclature with the point of cleavage indicated by a "/". Numbers in parentheses indicate distance from the recognition sequence.

For example, GGTCTC(1/5) indicates cleavage at:
5'... GGTCTCN ...3'
3'... CCAGAGNNNNN/ ...5'

EnzymeRecognition Sequence
ΔAhdIGACNNN/NNGTC
AlfI(10/12)GCANNNNNNTGC(12/10)
AgsITTS/AA
ΔAleICACNN/NNGTG
ΔAlwNICAGNNN/CTG
XApaBIGCANNNNN/TGC
ΔApeKIG/CWGC
ΔAvaIIG/GWCC
ΔBglIGCCNNNN/NGGC
BisIGC/NGC
ΔBlpIGC/TNAGC
BlsIGCN/GC
ΔBsaBIGATNN/NNATC
ΔRsrIICG/GWCCG
ΔBsaJIC/CNNGG
ΔBsaWIW/CCGGW
ΔBsiHKAIGWGCW/C
ΔBslICCNNNNN/NNGG
ΔBssKI/CCNGG
ΔBstAPIGCANNNN/NTGC
ΔBstEII  §G/GTNACC
ΔBstNICC/WGG
ΔBstXICCANNNNN/NTGG
ΔBsu36ICC/TNAGG
XBthCIGCNG/C
BplI(8/13)GAGNNNNNCTC(13/8)
BmgT120IGG/NCC
XCjuICAYNNNNNRTG
ΔCac8IGCN/NGC
ΔDdeIC/TNAG
ΔDraIII §CACNNN/GTG
ΔDrdIGACNNNN/NNGTC
XEcoHI/CCSGG
ΔEcoNICCTNN/NNNAGG
ΔEcoO109IRG/GNCCY
FalI(8/13)AAGNNNNNCTT(13/8)
XFmuIGGNC/C
XHaeIWGG/CCW
ΔHinfIG/ANTC
ΔHpy99ICGWCG/
ΔHpy188ITCN/GA
ΔHpy188IIITC/NNGA
ΔHpy166IIGTN/NAC
ΔHpyCH4IIIACN/GT
KflIGG/GWCCC
MaeIII/GTNAC
XMjaIVGTNNAC
ΔMslICAYNN/NNRTG
ΔMwoIGCNNNNN/NNGC
ΔNciICC/SGG
ΔNlaIVGGN/NCC
XPasICC/CWGGG
PcsIWCGNNNN/NNNCGW
ΔPflFIGACN/NNGTC
PfoIT/CCNGGA
ΔPflMICCANNNN/NTGG
ΔPpuMIRG/GWCCY
ΔPshAIGACNN/NNGTC
ΔPspGI/CCWGG
XPspO3IGGWC/C
XPssIRGGNC/CY
ΔSau96IG/GNCC
ΔScrFICC/NGG
SetIASST/
ΔSexAIA/CCWGGT
ΔSfiIGGCCNNNN/NGGCC
XSse8647IAG/GWCCT
ΔStyD4I/CCNGG
ΔStyI §C/CWWGG
ΔTfiIG/AWTC
TatIW/GTACW
ΔTseIG/CWGC
ΔTsp45I/GTSAC
ΔTspRINNCASTGNN/
XTssIGAGNNNCTC
ΔTth111IGACN/NNGTC
XUnbI/GGNCC
XVpak11AI/GGWCC
ΔXcmICCANNNNN/NNNNTGG
ΔXmnIGAANN/NNTTC

Enzymes marked with a (Δ) are available from NEB.
Enzymes marked with an (X) are not currently commercially available.
§ A HF version of this enzyme is available.