All recognition sequences are written 5' to 3' using the single letter code nomeclature with the point of cleavage indicated by a "/".
Numbers in parentheses indicate distance from the recognition sequence.
For example, GGTCTC(1/5) indicates cleavage at:
5'... GGTCTCN ...3'
3'... CCAGAGNNNNN/ ...5'
Enzymes marked with a (Δ) are available from NEB.
Enzymes marked with an (X) are not currently commercially available.
(1) A HF version of this enzyme is available.
(2) A RE-Mix version of this enzyme is available.