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Characteristics of Select E.coli Strains

The table summarizes relevant characteristics and recommended applications for each strain. Strain genotypes and brief descriptions are listed below. Genotypes of E. coli strains sold by NEB as ready-to-use competent cells can be found in Competent E. coli Strains from NEB.

  Library
Construc
-tion1
Plasmid
Prepara
-tion2
Cloning/
Sub-
cloning
Dam-/
Dcm-
Single-
stranded
Phage3
Blue/
White
Screening
RecA- Protease-
deficient4
Laclq
(for P lac
regulation)
Kunkel
Mutage-
nesis
Drug
Resistance5
ER1793 + + str
ER1821 + + + none
ER2267 + + + + + + + kan
ER2738 + + + + tet
ER2925(6) + + cam 
str
JM101 + + + none
JM109 + + + + + + nal
NM522 + + + + none
ER2507 + kan
str
TB1 + + str
CAG597 + tet
str
CAG626 + str
CAG629 + tet 
str
PR1031 + tet
ER2508 + kan
str
tet
KS1000 + + + kan
nal
tet
UT5600 + str
CJ236 + + cam

Notes

  1. Restriction-deficient strain.
  2. Strain has a mutation in the endA gene which eliminates the major nonspecific endonuclease.
  3. Strain contains F´.
  4. See strain description for details.
  5. cam = chloramphenicol; kan = kanamycin; nal = nalidixic acid; str = streptomycin; tet = tetracycline
  6. Also sold as ready-to-use competent cells (NEB #C2925H).
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