Blunting Selection Chart

T4 DNA
Polymerase*
(NEB #M0203)
DNA Polymerase I,
Large (Klenow)
Fragment
(NEB #M0210)
Quick Blunting
Kit
(NEB #E1201)
Mung Bean
Nuclease
(NEB #M0250)
APPLICATION
Removal of 3´ overhangs



Removal of 5´ overhangs



Fill in of 5´ overhangs




* T4 DNA Polymerase has a strong 3´→ 5´ exo activity