Blunting Selection Chart

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  • T4 DNA
    Polymerase*
    (NEB #M0203)
    DNA Polymerase I,
    Large (Klenow)
    Fragment
    (NEB #M0210)
    Quick Blunting
    Kit
    (NEB #E1201)
    Mung Bean
    Nuclease
    (NEB #M0250)
    APPLICATION
    Removal of 3´ overhangs



    Removal of 5´ overhangs



    Fill in of 5´ overhangs




    * T4 DNA Polymerase has a strong 3´→ 5´ exo activity