Interactive Tools

NEB Tools

  • NEBNext Selector
    NEBNext Selector
    Use this tool to guide you through selection of NEBNext reagents for next generation sequencing sample preparation.
  • $name
    Thermostable Ligase Reaction Temperature Calculator
    This tool will estimate an optimal reaction temperature to minimize mismatch for thermostable ligation of two adjacent ssDNA probes annealed to a template.
  • NEBcloner
    NEBcloner
    Use this tool to find the right products and protocols for each step (digestion, end modification, ligation and transformation) of your next traditional cloning experiment. Also, find other relevant tools and resources to enable protocol optimization.
  • NEBioCalculator
    NEBioCalculator
    Use this tool for your scientific calculations and conversions for DNA and RNA. Options include conversion of mass to moles, ligation amounts, conversion of OD to concentration, dilution and molarity.
  • $name
    NEBuilder Assembly Tool
    NEBuilder Assembly Tool can be used to design primers for your Gibson Assembly reaction, based on the entered fragment sequences and the polymerase being used for amplification.
  • Enzyme Finder
    Enzyme Finder
    Use this tool to select restriction enzymes by name, sequence, overhang or type. Enter your sequence using single letter code nomenclature, and Enzyme Finder will identify the right enzyme for the job.
  • Double Digest Finder
    Double Digest Finder
    Use this tool to guide your reaction buffer selection when setting up double-digests, a common timesaving procedure. Choosing the right buffers will help you to avoid star activity and loss of product.
  • DNAsequenceMapWebToolNEB
    DNA Sequences and Maps Tool
    Use this tool to find the nucleotide sequence files for commonly used molecular biology tools, including plasmid, viral and bacteriophage vectors.
  • PCR Selector
    PCR Selector
    Use this tool to help select the right DNA polymerase for your PCR setup. Whether your amplicon is long, complex, GC-rich or present in a single copy, the PCR selection tool will identify the perfect DNA polymerase for your reaction.
  • Tm Calculator
    Tm Calculator
    Use this tool when designing PCR reaction protocols to help determine the optimal annealing temperature for your amplicon. Simply input your DNA polymerase, primer concentration and your primer sequence and the Tm Calculator will guide you to successful reaction conditions.
  • NEB Cutter®
    NEBcutter V2.0
    Use this tool to identify the restriction sites within your DNA sequence. Choose between Type II and commercially available Type III restriction enzymes to digest your DNA. NEBcutter V2.0 will indicate cut frequency and methylation state sensitivity.
  • $name
    NEB Golden Gate Assembly Tool
    Use this tool to assist with in silico DNA construct design for Golden Gate DNA assembly. It enables the accurate design of primers with appropriate type IIS restriction sites and overlaps, quick import of sequences in many formats and export of the final assembly, primers and settings.
  • $name
    NEBaseChanger
    NEBaseChanger can be used to design primers specific to the mutagenesis experiment you are performing using the Q5 Site-Directed Mutagenesis Kit. This tool will also calculate a recommended custom annealing temperature based on the sequence of the primers by taking into account any mismatches.
  • $name
    NEBnow Locator
    Use this tool to find an NEBNow Freezer Program near you. Search by city, company or institution.

Additional Links

  • $name
    REBASE
    Use this tool as a guide to the ever-changing landscape of restriction enzymes. REBASE, the Restriction Enzyme DataBASE, is a dynamic, curated database of restriction enzymes and related proteins.
  • PolBase
    Polbase
    Polbase is a repository of biochemical, genetic, and structural information about DNA Polymerases.