NEB scientists are often involved in the development of online tools that will aid in their research. We are now making these tools, and, in some circumstances, the source code, available for you to evaluate. These tools are not yet optimized for design and usability, so we are looking for your feedback on their functionality and utility to help us improve them for future use.
Virtually digest a DNA sequence with each of the known Type II restriction enzymes
Genomes and Restriction Enzyme Frequencies
A resource detailing restriction enzyme frequencies (number of cuts) in various genomes
Ligase Fidelity Viewer
Visualize overhang ligation preferences to facilitate the design of high-fidelity Golden Gate assemblies
Please share your thoughts on how these tools and/or their code are working for you. Additionally, if you have any suggestions for new online tools that would help your research, please let us know. View NEB’s full list of online tools and mobile apps.Share Feedback
Use Restriction; The tools are for internal research only. No commercial use, reproduction or distribution of the tools is permitted. You shall not cause or allow the reverse engineering, disassembly, or decompilation of a tool.
Disclaimer of Liability; Limitation of Liability: THE TOOLS ARE EXPERIMENTAL IN NATURE AND PROVIDED “AS IS” WITHOUT ANY EXPRESS OR IMPLIED WARRANTY OF ANY KIND. IF YOU USE A TOOL, YOU, ON BEHALF OF THE COMPANY OR INSTITUTION YOU REPRESENT, ASSUME THE ENTIRE RISK REGARDING USES, OUTPUTS, ANALYSIS, RESULTS AND PERFORMANCE OF THE TOOL.
Feedback. Any feedback you provide regarding your use of a tool may be used by NEB for any purpose.