Protocol for Topoisomerase I (E. coli) (M0301)

1) Reaction Set up:

DNA Up to 1 µg
10X CutSmart® Buffer 5 µl
Topoisomerase I 1 µl (5 units)
H2O Up to 50 µl

2) Incubate at 37°C for 15 minutes.


Notes:

  1. The buffer is supplied with the reagent.