Typical RT-LAMP Protocol

  1. Component  Volume Final Concentration
    10X Isothermal Amplification Buffer  2.5 µl 1X
    MgSO4 (100 mM)  1.5 µl 6 mM (8 mM total)
    dNTP Mix (10 mM)  3.5 µl 1.4 mM
    FIP/BIP Primers (25X)  1.6 µl 1.6 µM
    F3/B3 Primers (25X)  0.2 µl 0.2 µM
    LoopF/B Primers (25X)  0.4 µl 0.4 µM
    Bst 2.0 or Bst 2.0
    WarmStart DNA Polymerase
     1 µl 8 units
    WarmStart RTx Reverse Transcriptase  0.5 µl 7.5 units
    Template  X µl  
    Total Reaction Volume  25 µl 25 µl

  2. Mix by vortexing.

  3. Place directly at 65°C.