Standard PCR Protocol (E5315)

CYCLE STEP TEMPERATURE TIME CYCLES
Reverse Transcription 48°C 15–30 minutes 1
Initial Denaturation 94°C 1 minute 1
Denaturation
Annealing
Extension
94°C
50–65°C
68°C
15 seconds
30 seconds
1 minute per kb

40
Final Extension 68°C 5 minutes 1
Hold 4°C 1