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  • Double Digest Protocol using Two RE-Mix® Enzymes


    1. Dilute up to 1 μg DNA to 36 μl with dH20
    2. Add 2 μl of each 10X RE-Mix
    3. Incubate for 15 minutes at 37°C
    4. Analyze by agarose gel electrophoresis

      Notes: Alternatively, double digest can be carried out in a 20 μl volume using 1 μl of each RE-Mix and a minimum incubation time of 30 minutes.

    Some Standard Restriction Enzymes are not compatible with RE-Mix

    Standard Restriction Enzymes Not Compatible with RE-Mix Master Mixes
    (Sequential Digestions Recommended)
    AleI DpnII SalI
    AlwI MluI SexAI
    BanI MwoI SfaNI
    BciVI NmeAIII SgrAI
    BfaI NotI SphI-HF
    BspCNI PacI StyI
    BspEI PvuI StyD4I
    BtgZI RsrII Tsp45I