• My NEB
  • Print
  • PDF
  • Double Digest Protocol using Two RE-Mix® Enzymes


    1. Dilute up to 1 μg DNA to 36 μl with dH20
    2. Add 2 μl of each 10X RE-Mix
    3. Incubate for 15 minutes at 37°C
    4. Analyze by agarose gel electrophoresis

      Notes: Alternatively, double digest can be carried out in a 20 μl volume using 1 μl of each RE-Mix and a minimum incubation time of 30 minutes.
    There is also a protocol for double digest with a standard restriction enzyme with RE-Mix.