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  • Radioactive Labeling with T4 PNK or T4 PNK 3' phosphatase minus

    Introduction

    For radioactive labeling, use 1-50 pmol of 5' termini in a 50 µl reaction containing 1X T4 Polynucleotide Kinase Buffer, 50 pmol of [γ-32P] ATP and 20 units of T4 Polynucleotide Kinase. [33P] ATP may be substituted for [32P] ATP.