Catalog # S9236 was discontinued on December 15, 2022
BC-NH2 is an amine-terminated building block for the one-step synthesis of CLIP-tag™ substrates from NHS esters or other activated carboxylic esters of labels or surfaces.
This building block allows you to make custom CLIP-tag substrates for labeling CLIP-tag fusion proteins
It is particularly useful for the synthesis of fluorescent CLIP-tag substrates
Product Information
The CLIP-tag protein labeling system enables the specific, covalent attachment of virtually any molecule to a protein of interest. CLIP-tag is a protein tag based on human O6-alkylguanine-DNA-alkyltransferase (hAGT). CLIP-tag substrates are derivatives of benzylcytosine (BC). In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the reactive cysteine of CLIP-tag forming a stable thioether bond. Although CLIP-tag is based on the same protein as SNAP-tag®, the benzylcytosine substrates form a separate class of substrates, different from the benzylguanine substrates recognized by SNAP-tag. CLIP-tag and SNAP-tag can be used for orthogonal and complementary labeling of two proteins simultaneously in the same cells.
There are two steps to using this system: sub-cloning and expression of the protein of interest as a CLIP-tag fusion, and labeling of the fusion with the CLIP-tag substrate of choice. Expression of CLIP-tag fusion proteins is described in the documentation supplied with CLIP-tag plasmids. The labeling of the fusion proteins with the CLIP-tag substrate is described in this document.
Figure 1. Structure of BC-NH2 (MW 230.3 g/mol)
This product is related to the following categories:
The BC-NH2 building block is soluble in anhydrous DMF. Choose a solvent volume that will be compatible with the final use. Heating at 37°C, vortexing and/or sonication will aid dissolution. Unused BC-NH2 substrate dissolved in DMF should be stored at -20°C.
References
Gautier A. et al. (2008). Chemistry & Biology. 15, 128-136.
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Specifications & Change Notifications
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
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Licenses
Cellular Imaging and Analysis (i.e., SNAP and CLIP products)
Notice to Buyer/User: The Buyer/User has a non-exclusive license to use this system or any component thereof for RESEARCH AND DEVELOPMENT ONLY. Commercial use of this system or any components thereof requires a license from New England Biolabs, Inc., 240 County Road, Ipswich, MA 01938. For detailed information see our Terms of Use .
These patents and patent applications are owned by Covalys, or owned by the Ecole Polytechnique Fédérale de Lausanne (EPFL) and exclusively licensed to Covalys and NEB.
The products and/or their use may be covered by one or more of the following patents and patent applications: Labeling of Fusion Proteins with Synthetic Probes: US 8,227,602; US 8,623,627; and EP 2 049 499.
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