Thanksgiving Shipping Schedule: Orders placed on Tuesday, November 22, 2022, will ship for delivery Wednesday, November 23, 2022. We will be closed on Thursday November 24, 2022, and Friday, November 25, 2022.

Orders placed on Wednesday November 23rd, will be shipped on Monday, November 28th.

BC-NH2

This product will be discontinued on December 15, 2022.

BC-NH2 is an amine-terminated building block for the one-step synthesis of CLIP-tag™ substrates from NHS esters or other activated carboxylic esters of labels or surfaces.

  • This building block allows you to make custom CLIP-tag substrates for labeling CLIP-tag fusion proteins
  • It is particularly useful for the synthesis of fluorescent CLIP-tag substrates
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Catalog # Concentration Size List Price Your Price Quantity
S9236S Not Applicable 2 mg $426.00
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  • Product Information
    The CLIP-tag protein labeling system enables the specific, covalent attachment of virtually any molecule to a protein of interest. CLIP-tag is a protein tag based on human O6-alkylguanine-DNA-alkyltransferase (hAGT). CLIP-tag substrates are derivatives of benzylcytosine (BC). In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the reactive cysteine of CLIP-tag forming a stable thioether bond. Although CLIP-tag is based on the same protein as SNAP-tag®, the benzylcytosine substrates form a separate class of substrates, different from the benzylguanine substrates recognized by SNAP-tag. CLIP-tag and SNAP-tag can be used for orthogonal and complementary labeling of two proteins simultaneously in the same cells.

    There are two steps to using this system: sub-cloning and expression of the protein of interest as a CLIP-tag fusion, and labeling of the fusion with the CLIP-tag substrate of choice. Expression of CLIP-tag fusion proteins is described in the documentation supplied with CLIP-tag plasmids. The labeling of the fusion proteins with the CLIP-tag substrate is described in this document.

    Figure 1. Structure of BC-NH2 (MW 230.3 g/mol)
    Figure 1. Structure of BC-NH2 (MW 230.3 g/mol)

    This product is related to the following categories:
    Building Blocks Products,
    CLIP-tag™ Substrates Products,
    Protein Labeling Products
    This product can be used in the following applications:
    Protein Labeling Snap Clip,
    Cell Imaging
    • Reagents Supplied
    • Properties & Usage
    • Product Notes
    • References
  • Protocols, Manuals & Usage
  • Tools & Resources
  • FAQs & Troubleshooting
  • Citations & Technical Literature
  • Quality, Safety & Legal
    • Quality Control Assays
    • Specifications & Change Notifications
    • Certificate of Analysis
    • Safety Data Sheets
    • Legal and Disclaimers

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