BC-NH2 is an amine-terminated building block for the one-step synthesis of CLIP-tag™ substrates from NHS esters or other activated carboxylic esters of labels or surfaces.
There are two steps to using this system: sub-cloning and expression of the protein of interest as a CLIP-tag fusion, and labeling of the fusion with the CLIP-tag substrate of choice. Expression of CLIP-tag fusion proteins is described in the documentation supplied with CLIP-tag plasmids. The labeling of the fusion proteins with the CLIP-tag substrate is described in this document.
The following reagents are supplied with this product:
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