BG-NH2 is an amine-terminated building block for the one-step synthesis of SNAP-tag® substrates from NHS esters or other activated carboxyl esters of labels or surfaces.
- This compound allows you to make custom SNAP-tag substrates for labeling SNAP-tag fusion proteins for a wide range of applications
- BG-NH2 is particularly useful for the synthesis of fluorescent SNAP-tag substrates
The SNAP-tag protein labeling system enables the specific, covalent attachment of virtually any molecule to a protein of interest. The SNAP-tag is a protein based on human O6-alkylguanine-DNA-alkyltransferase (hAGT). SNAP-tag substrates are fluorophores, biotin or beads conjugated to guanine or chloropyrimidine leaving groups via a benzyl linker. In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the SNAP-tag.
There are two steps to using this system: subcloning and expression of the protein of interest as a SNAP-tag fusion, and labeling of the fusion with the SNAP-tag substrate of choice. Expression of SNAP-tag fusion proteins is described in the documentation supplied with SNAP-tag plasmids. The labeling of fusion proteins with the SNAP-tag substrate is described in this document.
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