Goat Anti-Rat IgG Magnetic Beads

Description

An affinity matrix for the small-scale immunomagnetic separation and purification of rat IgG’s. anti-Rat IgG is covalently coupled to a 1μm nonporous superparamagnetic particle. This secondary antibody binds the Fc portion of all monoclonal rat IgG subclasses and is suitable for immunoassays that employ a rat IgG primary monoclonal antibody. Cell separations and sorting can be accomplished using a rat IgG antibody to defined cell surface antigens.

Properties and Usage

Storage Temperature

4°C

Storage Conditions

0.05% Tween® 20
0.05 mM NaN3
1 ml suspension in PBS Buffer
pH 7.4 @ 25°C

Binding Capacity

5 ug Rat IgG / mg of Goat Anti-Rat IgG magnetic beads

Notes

  1. The amount of antibody required for optimal coating of the beads will vary with the antibody Ab affinity and the antigen density on the cell surface. Optimal experimental conditions should be determined by titration on an individual basis.

Protocols

  1. Goat Anti-Rat IgG Magnetic Beads (S1433)

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

Interactive Tools

Safety Data Sheet

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.