Hydrophilic Streptavidin Magnetic Beads

Description

Hydrophilic Streptavidin Magnetic Beads are 2 µm superparamagnetic particles covalently coupled to a highly pure form of streptavidin. The beads can be used to capture biotin labeled substrates including antigens, antibodies and nucleic acids (1,2). The strength of the biotin-streptavidin interaction, an association constant (Ka) of 1015 M-1 coupled with the low non-specific binding of streptavidin permits captured substrates to be useful as ligands in subsequent experiments including mRNA isolation and the capture of primary or secondary antibodies (3,4).

Beads are supplied as a 4 mg/ml suspension in a phosphate buffer (PBS) (pH 7.4) containing 0.1% BSA, 0.05% NaN3 and 0.05% Tween® -20.

Properties and Usage

Storage Temperature

4°C

Storage Conditions

0.1% BSA
0.05% Tween® 20
0.02% sodium azide
1X PBS
pH 7.4 @ 25°C

Binding Capacity

The beads will bind greater than 800 pmol of free biotin per mg and greater then 400 pmol of single-stranded 25 bp biotinylated oligonucleotide per mg.

Notes

  1. Beads will bind greater than 800 pmol of free biotin per mg and greater than 400 pmol of single-stranded 20 bp biotinylated oligonucleotide per mg or Hydrophilic Streptavidin Magnetic Beads.

References

  1. Chung, S. et al. (2005). J. Biol. Chem.. 280, 4578.
  2. Tang, B. et al. (2003). Genome. 46, 833--840.
  3. Zhang, X. et al. (2006). J. Clin. Invest.. 116, 3050-3059.
  4. Fernandez-Cabezudo, M.J. et al. (2004). Internat. Immunol.. 16, 1215-1223.

FAQs

  1. What is the difference between S1420 and S1421?

Protocols

  1. Phage Display: Solution-phase Panning with Affinity Bead Capture
  2. Streptavidin Magnetic Beads

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

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Safety Data Sheet

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.