G(5')ppp(5')G RNA Cap Structure Analog

  • Co-transcriptional capping with T7, SP6 and T3 RNA polymerases
  • Synthesis of unmethylated G-capped RNA
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Catalog # Concentration Size List Price Your Price Quantity
S1407S Not Applicable 1 µmol $119.00
S1407L Not Applicable 5 µmol $477.00
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  • Product Information
    The 5´ terminal m7G cap present on most eukaryotic mRNAs promotes translation in vitro at the initiation level (1,2,3). For most RNAs, elimination of the cap structure causes a loss of stability, especially against exonuclease degradation (4), and a decrease in the formation of the initiation complex of mRNAs for protein synthesis (4,5). Certain prokaryotic mRNAs containing a 5´ terminal cap structure are translated as efficiently as or more efficiently than eukaryotic mRNAs in a eukaryotic cell-free protein synthesizing system (5). Also a cap requirement has been observed for splicing eukaryotic substrate RNAs (6).

    A method using E. coli RNA Polymerase primed with m7G(5´)ppp(5´)G or m7G(5´)ppp(5´)A for an efficient in vitro synthesis of capped RNAs has been developed by Contreas (7). Larger amounts of capped RNAs are produced by transcription systems using SP6 RNA polymerase primed with m7G(5´)ppp(5´)G (6).

    Sequence

    G(5')ppp(5')G
    Product Categories:
    RNA Synthesis Products
    Applications:
    RNA Modification,
    In vitro Synthesis (IVT)
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