NEB offers several RNA Markers and Ladders with a size range from 17 to 9,000 bases. The Low Range ssRNA Ladders (NEB #N0364) are suitable for use as RNA size standards on denaturing or native gels. Both are supplied with 2X Sample Buffer and feature a double intensity fragment to serve as a reference band. The microRNA Marker (NEB #N2102) provided in a ready-to-load denaturing solution, is ideally used as a size marker on denaturing polyacrylamide gels or Northern blots and is best visualized stained with SYBR-Gold. It is supplied with a 3´-biotinylated 21-mer oligonucleotide probe that can be labeled with γ32-P-ATP using T4 PNK (NEB #M0201). The dsRNA Ladder (NEB #N0363) is suitable for use as a size standard in dsRNA and RNAi analysis on both denaturing polyacrylamide and agarose gels. The siRNA Marker (NEB #N2101) can be used as an siRNA size standard on native polyacrylamide gels.
- Avoiding Ribonuclease Contamination
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