NEB offers several RNA Markers and Ladders with a size range from 17 to 9,000 bases. The Low Range ssRNA Ladders (NEB #N0364) are suitable for use as RNA size standards on denaturing or native gels. Both are supplied with 2X Sample Buffer and feature a double intensity fragment to serve as a reference band. The microRNA Marker (NEB #N2102) provided in a ready-to-load denaturing solution, is ideally used as a size marker on denaturing polyacrylamide gels or Northern blots and is best visualized stained with SYBR-Gold. It is supplied with a 3´-biotinylated 21-mer oligonucleotide probe that can be labeled with γ32-P-ATP using T4 PNK (NEB #M0201). The dsRNA Ladder (NEB #N0363) is suitable for use as a size standard in dsRNA and RNAi analysis on both denaturing polyacrylamide and agarose gels. The siRNA Marker (NEB #N2101) can be used as an siRNA size standard on native polyacrylamide gels.
FAQs for RNA Markers & Ladders
Protocols for RNA Markers & Ladders
- Avoiding Ribonuclease Contamination
Other Tools & Resources
RNA Reagents Overview
Reagents for RNA Sample Preparation
RNA Polymerase Selection Chart
RNA Ligase Selection Chart
cDNA Synthesis Selection Chart
While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
For more information about commercial rights, please contact NEB's Global Business Development team at firstname.lastname@example.org.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.