RNA Markers & Ladders

NEB offers several RNA Markers and Ladders with a size range from 17 to 9,000 bases. The Low Range ssRNA Ladders (NEB #N0364) are suitable for use as RNA size standards on denaturing or native gels. Both are supplied with 2X Sample Buffer and feature a double intensity fragment to serve as a reference band. The microRNA Marker (NEB #N2102) provided in a ready-to-load denaturing solution, is ideally used as a size marker on denaturing polyacrylamide gels or Northern blots and is best visualized stained with SYBR-Gold. It is supplied with a 3´-biotinylated 21-mer oligonucleotide probe that can be labeled with γ32-P-ATP using T4 PNK (NEB #M0201). The dsRNA Ladder (NEB #N0363) is suitable for use as a size standard in dsRNA and RNAi analysis on both denaturing polyacrylamide and agarose gels. The siRNA Marker (NEB #N2101) can be used as an siRNA size standard on native polyacrylamide gels.

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FAQs for RNA Markers & Ladders
Protocols for RNA Markers & Ladders
RNA Reagents Overview
Reagents for RNA Sample Preparation
NEB offers a wide variety of reagents to facilitate front-end processing of RNA for downstream applications including next-generation sequencing, library construction and microarrays.
RNA Polymerase Selection Chart
NEB offers RNA polymerases that can be used for in vitro synthesis of RNA for a wide variety of downstream applications. Additional polymerases are offered for the generation of untemplated homoribopolymeric tails for reverse transcription or labeling.
RNA Ligase Selection Chart
NEB offers a variety of ligases for RNA research with unique specificities.
cDNA Synthesis Selection Chart
NEB offers several reagents for cDNA synthesis for use in applications including qPCR and qRT-PCR. For your convenience, reagents are available as kits or standalone products, depending on your needs.