p-Nitrophenyl Phosphate (PNPP)

p-Nitrophenyl Phosphate (PNPP) is a non-proteinaceous, non-specific substrate used to assay protein, alkaline and acid phosphatases.

  • Can be used for quick analysis of the protein phosphatase activity under any conditions 
  • Advantageous to a radioactive assay because the substrate concentration can be much higher than the Km
  • Activity is measured using a continuous or single-point spectrophotometric assay based on the ability of phosphatases to catalyze the hydrolysis of PNPP to p-nitrophenol, a chromogenic product that absorbs maximally at 405 nm
Learn more about how this product is being used in the Product Citation Tool
2019-20_Catalog_Promo_0519
Catalog # Concentration Size List Price Your Price Quantity
P0757S 500 mM 1 ml $32.00
P0757L 500 mM 5 ml $128.00
Please enter a quantity for at least one size

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