Home DNA Plasmids & Substrates ΦX174 Virion DNA

ΦX174 Virion DNA

ΦX174 Virion DNA is the single-stranded viral DNA isolated from bacteriophage ΦX174 am3 cs70.
Molecular weight: 1.70 x 10⁶ daltons
Size: 5386 bases

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Concentration

1,000 μg/ml

Size

50 μg

List Price

$77.00

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Concentration

1,000 μg/ml

Size

250 μg

List Price

$308.00

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Product Information
ΦX174 Virion DNA is the single-stranded viral DNA isolated from bacteriophage ΦX174 am3 cs70. It has a molecular weight of 1.70 x 10⁶ daltons and is 5386 bases in length. Greater than 85% of the molecules are circular.
DNASU and Addgene are central repositories for plasmid clones and collections that may also be helpful.

Product Source
The phage is grown in E. coli HF4704 and purified by cesium chloride density gradient centrifugation. The DNA is isolated from the purified phage by phenol extraction according to the procedure of Sinsheimer.

Product Categories:

DNA Plasmids & Substrates
- Properties and Usage
  
  Storage Temperature
  -20°C
  Storage Conditions
  
  10 mM Tris-HCl
  1 mM EDTA
  pH 8.0 @ 25°C
- Related Products
  Companion Products
  
  фX174 RF I DNA
  
  фX174 RF II DNA
  
  Monarch® Plasmid Miniprep Kit
  
  Monarch® DNA Gel Extraction Kit
  
  Monarch® PCR & DNA Cleanup Kit (5 μg)
- References
  Sanger, F., Coulson, A.R., Friedmann, T., Air, G.M., Barrell, B.G., Brown, N.L., Fiddes, J. C., Hutchison, C.A. III, Slocombe, P.M. and Smith, M. (1978). J. Mol. Biol.. 125, 225-246.
  
  Sinsheimer, R.L. (1966). Cantoni and Davies(Ed.), Procedures in Nucleic Acid Research. 569. Harper and Row.
FAQs & Tech Tips
- Tech Tips
  
  φhiX174 is available in different forms: single-stranded (from phage particles) or double-stranded (from phage infected E.coli). Generally, single-stranded DNAs are not cut by restriction enzymes.
Protocols & Manuals
- Datacards
  The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.)
  
  N3023Datasheet-Lot1221204
  
  N3023Datasheet-Lot1231209
  
  N3023Datasheet-Lot1231302
  
  N3023Datasheet-Lot1231401
  
  N3023Datasheet-Lot1241407
  
  N3023Datasheet-Lot1241412
  
  N3023Datasheet-Lot1241505
  
  N3023Datasheet-Lot1241510
  
  N3023Datasheet-Lot1241604
  
  N3023Datasheet-Lot1251608
  
  N3023Datasheet-Lot1251612
Other Tools & Resources
- Interactive Tools
  DNA Sequences and Maps Tool
  
  NEBioCalculator
Quality & Safety
- Quality Control Assays
  
  Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
- Specifications
  The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
  
  N3023S_L_v1
- Certificate of Analysis
  The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
  
  N3023S_L_v1_1241407
  
  N3023S_L_v1_1251704
  
  N3023S_L_v1_1261712
  
  N3023L_v1_10014944
  
  N3023S_v1_10014946
- Safety Data Sheets
  The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.
  
  ΦX174 Virion DNA
- Datacards
  The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.)
  
  N3023Datasheet-Lot1221204
  
  N3023Datasheet-Lot1231209
  
  N3023Datasheet-Lot1231302
  
  N3023Datasheet-Lot1231401
  
  N3023Datasheet-Lot1241407
  
  N3023Datasheet-Lot1241412
  
  N3023Datasheet-Lot1241505
  
  N3023Datasheet-Lot1241510
  
  N3023Datasheet-Lot1241604
  
  N3023Datasheet-Lot1251608
  
  N3023Datasheet-Lot1251612
Legal Information
- Legal And Disclaimers
  
  This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).
  
  While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
  
  For more information about commercial rights, please contact NEB's Global Business Development team at gbd@neb.com.
  
  This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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Applications

Product Categories

You’ll be Thrilled to Pieces

Tools

Resources

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ΦX174 Virion DNA

Ordering Information

Product Source

Storage Temperature

Storage Conditions

Companion Products

Also Recommended

ΦX174 RF II DNA

ΦX174 RF I DNA

ΦX174 Virion DNA

Ordering Information

Product Source

Storage Temperature

Storage Conditions

Companion Products

Also Recommended

ΦX174 RF II DNA

ΦX174 RF I DNA

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