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    фX174 Virion DNA

    Description

    ΦX174 Virion DNA is the single-stranded viral DNA isolated from bacteriophage ΦX174 am3 cs70. It has a molecular weight of 1.70 x 106 daltons and is 5386 bases in length. Greater than 85% of the molecules are circular.

    DNASU is a central repository for plasmid clones and collections that may also be helpful.

    Product Source

    The phage is grown in E. coli HF4704 and purified by cesium chloride density gradient centrifugation. The DNA is isolated from the purified phage by phenol extraction according to the procedure of Sinsheimer.

    Properties and Usage

    Affinity Tag

    Other

    Storage Temperature

    -20°C

    Storage Conditions

    10 mM Tris-HCl
    1 mM EDTA
    pH 8.0 @ 25°C

    References

    1. Sanger, F., Coulson, A.R., Friedmann, T., Air, G.M., Barrell, B.G., Brown, N.L., Fiddes, J. C., Hutchison, C.A. III, Slocombe, P.M. and Smith, M. (1978). J. Mol. Biol.. 125, 225-246.
    2. Sinsheimer, R.L. (1966). Cantoni and Davies(Ed.), Procedures in Nucleic Acid Research. 569. Harper and Row.

    Supporting Documents

    Material Safety Datasheets

    The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

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