ΦX174 Virion DNA

Description

ΦX174 Virion DNA is the single-stranded viral DNA isolated from bacteriophage ΦX174 am3 cs70. It has a molecular weight of 1.70 x 106 daltons and is 5386 bases in length. Greater than 85% of the molecules are circular.

DNASU and Addgene are central repositories for plasmid clones and collections that may also be helpful.

Product Source

The phage is grown in E. coli HF4704 and purified by cesium chloride density gradient centrifugation. The DNA is isolated from the purified phage by phenol extraction according to the procedure of Sinsheimer.

Properties and Usage

Storage Temperature

-20°C

Storage Conditions

10 mM Tris-HCl
1 mM EDTA
pH 8.0 @ 25°C

References

  1. Sanger, F., Coulson, A.R., Friedmann, T., Air, G.M., Barrell, B.G., Brown, N.L., Fiddes, J. C., Hutchison, C.A. III, Slocombe, P.M. and Smith, M. (1978). J. Mol. Biol.. 125, 225-246.
  2. Sinsheimer, R.L. (1966). Cantoni and Davies(Ed.), Procedures in Nucleic Acid Research. 569. Harper and Row.

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

Interactive Tools

Certificate of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality control's for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Safety Data Sheet

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.