1 kb Plus DNA Ladder for Safe Stains

Reagents Supplied

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
N0559S     4       1 kb Plus DNA Ladder for Safe Stains N0559SVIAL 4 1 x 1.25 ml 50 µg/ml

Companion Products

• Quick-Load® Purple 1 Kb Plus DNA Ladder
• 1 kb Plus DNA Ladder
• Monarch® Plasmid Miniprep Kit 
• Monarch® DNA Gel Extraction Kit
• Monarch® PCR & DNA Cleanup Kit (5 μg)

1. Preparation of DNA:
   The double-stranded DNA is digested to completion with appropriate restriction enzymes, phenol extracted and equilibrated in storage buffer.
2. Usage recommendation:
   We recommend loading 5 µl (0.25 µg) of 1 kb Plus DNA Ladder for Safe Stains per gel lane on GelRed^® and GelGreen^® precast agarose gels. We recommend loading 10 µl (0.5 µg) of 1 kb Plus DNA Ladder for Safe Stains per gel lane on SYBR^® Safe precast agarose gels. 
3. Storage Recommendations:
   1 kb Plus DNA Ladder for Safe Stains is stable for at least 6 months at 25°C. For long term storage, store at 4°C or -20°C. If stored at -20°C, mix well after thawing. If not well mixed, the load may float out of the wells.
4. Usage Recommendations:
   • The 1 kb Plus DNA Ladder for Safe Stains is not intended for precise quantification of DNA mass but can be used for approximating the mass of DNA in comparably intense samples of similar size.
   • The 1 kb Plus DNA Ladder for Safe Stains might be used on SYBR Green I precast agarose gels but might require further dilution and optimization. We recommend using SYBR Green I as a post-electrophoresis stain for best results.
   • We recommend using SYBR Gold as a post-electrophoresis stain only, as recommended by the dye’s manufacturer.
   • The 1 kb Plus DNA Ladder for Safe Stains is not intended for use on short mini-gels or fast electrophoresis systems. We recommend a minimum of 7 cm of gel length for proper and efficient separation. Best results occur with migration at 5 V/cm (distance between electrodes) for 60 to 90 minutes.
   • Most gel stains run in the opposite direction from that of the migrating DNA. As a result, the bottom of the precast gel might have a lower concentration of stain, which might result in un-uniform staining of the bottom bands. The length of the gel that will contain a lower stain concentration is highly dependent on the migration conditions (gel percentage, voltage, buffer, run time, etc.). This effect can be counteracted by adding some gel stain to the running buffer.

1. Sambrook, J., Fritsch, E. F. and Maniatis, T. (1989). Molecular cloning: A Laboratory Manual, (2nd ed.). 10.51-10.67. Cold Spring Harbor: Cold Spring Harbor Laboratory Press.

1. Protocol for loading 1 kb Plus DNA Ladder for Safe Stains (NEB #N0559)

• DNA Markers & Ladders Selection Chart

1. How much of the 1 kb Plus DNA Ladder for Safe Stains should I load?
2. Can I use the 1 kb Plus DNA Ladder for Safe Stains with SYBR^® Green?
3. Can I use the 1 kb Plus DNA Ladder for Safe Stains with SYBR^® Gold?
4. Why are the some of the bands in the DNA Ladder fainter at the bottom of the gel?
5. Can I use the 1 kb Plus DNA Ladder for Safe Stains on mini-gels or fast electrophoresis systems?

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications & Change Notifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• N0559S_v1

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• N0559G_v0_10046178
• N0559S_v1_10046179
• N0559S_v1_10066412
• N0559S_v1_10077707
• N0559S_v1_10085604
• N0559S_v1_10100171
• N0559S_v1_10118733
• N0559S_v1_10142927
• N0559S_v1_10158758
• N0559S_v1_10176555
• N0559S_v1_10182187

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• 1 kb Plus DNA Ladder for Safe Stains

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.