dUTP Solution

  • ≥ 99% purity as determined by HPLC analysis
  • For use with polymerases such as Taq DNA Polymerase (NEB# M0273) or Q5U® Hot Start High-Fidelity DNA Polymerase (NEB# M0515)
  • Combine with Antarctic Thermolabile UDG (NEB# M0372) to prevent carryover contamination in PCR and qPCR-related technologies
Catalog # Concentration Size List Price Your Price Quantity
N0459S 100 mM 25 µmol $62.00
Please enter a quantity for at least one size
  • Product Information

    Contains 0.25 ml of 100 mM ultrapure dUTP. dUTP can either be fully substituted for dTTP or added in addition to conventional dNTPs. dUTP can be used with Family A polymerases (such as Taq) or modified Family B polymerases, such as Q5U Hot Start High-Fidelity DNA Polymerase. Q5U contains a mutation in the uracil-binding pocket that enables the ability to read and amplify templates containing uracil and inosine bases.

    For a simplified protocol, dUTP can be added to most polymerase reactions at the same concentration as other nucleotides (e.g., a 1:1:1:1:1 dA/dC/dG/dT/dU ratio). 

    For applications that require other molar ratios (up to and including full substitution of dU for dT), custom nucleotide mixes must be made. We recommend starting with a concentration of 0.2 mM of each dNTP for both Taq and Q5U DNA polymerases.

    Supplied in: 
    Ultra purified water as a sodium salt at pH 7.5.

    The dUTP Solution is supplied at a concentration of 100 mM (+/- 5 mM) as determined by A260 absorbance.

    Diluent Compatibility: 
    Can be diluted using sterile distilled water, preferably Milli-Q® water or can be diluted using sterile TE [10 mM Tris-HCl, 1 mM EDTA (pH 7.5)].


    Reagents Supplied

    The following reagents are supplied with this product:

    NEB # Component Name Component # Stored at (°C) Amount Concentration
    Product Categories:
    Nucleotide Solutions Products,
    PCR, qPCR & Amplification Technologies Products
    USER® Cloning,
    Applications of USER® and Thermolabile USER II Enzymes,
    High-Fidelity PCR,
    DNA Amplification, PCR & qPCR,
    Specialty PCR,
    Routine PCR,
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