dUTP Solution
- ≥ 99% purity as determined by HPLC analysis
- For use with polymerases such as Taq DNA Polymerase (NEB# M0273) or Q5U® Hot Start High-Fidelity DNA Polymerase (NEB# M0515)
- Combine with Antarctic Thermolabile UDG (NEB# M0372) to prevent carryover contamination in PCR and qPCR-related technologies
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Product Information
Contains 0.25 ml of 100 mM ultrapure dUTP. dUTP can either be fully substituted for dTTP or added in addition to conventional dNTPs. dUTP can be used with Family A polymerases (such as Taq) or modified Family B polymerases, such as Q5U Hot Start High-Fidelity DNA Polymerase. Q5U contains a mutation in the uracil-binding pocket that enables the ability to read and amplify templates containing uracil and inosine bases.
For a simplified protocol, dUTP can be added to most polymerase reactions at the same concentration as other nucleotides (e.g., a 1:1:1:1:1 dA/dC/dG/dT/dU ratio).
For applications that require other molar ratios (up to and including full substitution of dU for dT), custom nucleotide mixes must be made. We recommend starting with a concentration of 0.2 mM of each dNTP for both Taq and Q5U DNA polymerases.Supplied in:
Ultra purified water as a sodium salt at pH 7.5.
Concentration:
The dUTP Solution is supplied at a concentration of 100 mM (+/- 5 mM) as determined by A260 absorbance.
Diluent Compatibility:
Can be diluted using sterile distilled water, preferably Milli-Q® water or can be diluted using sterile TE [10 mM Tris-HCl, 1 mM EDTA (pH 7.5)].- This product is related to the following categories:
- Nucleotide Solutions Products,
- PCR, qPCR & Amplification Technologies Products
- This product can be used in the following applications:
- USER® Cloning,
- Applications of USER® and Thermolabile USER II Enzymes,
- High-Fidelity PCR,
- Specialty PCR,
- DNA Amplification, PCR & qPCR,
- Routine PCR, PCR
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