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  • NEBNext® RNA First Strand Synthesis Module

    Description

    The NEBNext RNA First Strand Synthesis Module is designed for synthesis of cDNA from RNA fragments using ProtoScript II Reverse Transcriptase and random priming. Single strand cDNA can be used directly for second strand cDNA synthesis.

    Functional Validation

    Each set of reagents is functionally validated together through construction and sequencing of a transcriptome library and sequenced on a Illumina.

    Lot Control

    The lots provided are managed separately and qualified by additional functional validation. Individual reagents undergo standard enzyme activity and quality control assays, and also meet stringent criteria in the additional quality controls listed on each individual component page

    Reagents Supplied

    The following reagents are supplied with this product:

    Store at (°C)Concentration
    NEBNext First Strand Synthesis Reaction Buffer-205X
    Random Primers-20
    ProtoScript® II Reverse Transcriptase-20200,000 units/ml
    Murine RNase Inhibitor-20

    Properties and Usage

    Materials Required but not Supplied

    NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #E7490)

    If you are using the module for Directional RNA Seq, Actinomycin D (Sigma #A1410 dissolved in dimethylsulfoxide [DMSO] to 5μg/μl) is required. See protocol for details.

    Storage Temperature

    -20°C

    Supporting Documents

    Material Safety Datasheets

    The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.

    Manuals

    The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name these document files: manual[Catalog Number].

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.
    1. What other materials do I need in addition to the NEBNext First Strand Synthesis Module?
    2. What if I have already fragmented my mRNA and just need to perform the First Strand Synthesis Reaction?
    1. Protocol for Use with Total RNA (E7525)
    2. Protocol for Use with Previously Fragmented RNA (E7525)