NEBNext® Multiplex Small RNA Library Prep Set for SOLiD™ (Set 1)

This product has been discontinued. NEBNext® Small RNA Library Prep Set for SOLiD™ (E6160S/L) is a suitable alternative for most applications.

  • This product was discontinued on July 11, 2014

Ordering Information

E7450
  • Discontinued
    Discontinued
  • Product Information
    The NEBNext Multiplex Small RNA Library Prep Set for SOLiD (Set 1) contains adaptors, primers, enzymes and buffers that are ideal to convert small RNA into cDNA libraries for next-generation sequencing on the SOLiD platform (Life Technologies, Inc.). Each of these components must pass rigorous quality control standards and are lot controlled, both individually and as a set of reagents.

    Small RNA Library Prep Workflow
    Novel Protocol that Results in Higher Yields and Lower Adaptor-dimer Contamination (Patent Pending):
    E7450
    Functional Validation
    Each set of reagents is functionally validated together through construction and sequencing of an RNA library on the SOLiD™ 4.

    GenomeWeb article reviews NEBNext Small RNA Kits.

    Lot Control

    The lots provided are managed separately and qualified by additional functional validation. Individual reagents undergo standard enzyme activity and quality control assays, and also meet stringent criteria in the additional quality controls listed on each individual component page

    Reagents Supplied

    The following reagents are supplied with this product:

    Store at (°C) Concentration
    NEBNext 3´ Ligation Reaction Buffer -20 2 X
    NEBNext 3´ Ligation Enzyme Mix -20
    Murine RNase Inhibitor -20
    NEBNext 3´ SR Adaptor 3 -20
    NEBNext 5´ SR Adaptor 3 -20
    NEBNext 5´ Ligation Reaction Buffer -20 10 X
    NEBNext 5´ Ligation Enzyme Mix -20
    NEBNext SR RT Primer 3 -20
    OneTaq Hot Start 2X Master Mix with Standard Buffer -20 2 X
    NEBNext SR Primer R3 -20
    Gel Loading Dye, Blue 25 6 X
    Quick-Load pBR322 DNA-MspI Digest -20 100 μg/ml
    DNA Gel Elution Buffer 4 1 X
    Linear Acrylamide -20 10 mg/ml
    Nuclease-free Water -20
    TE Buffer -20
    ProtoScript® II Reverse Transcriptase -20 200,000 units/ml
    NEBNext First Strand Synthesis Reaction Buffer -20 5 X
    NEBNext Index 1 Primer for SOLiD -20
    NEBNext Index 2 Primer for SOLiD -20
    NEBNext Index 3 Primer for SOLiD -20
    NEBNext Index 4 Primer for SOLiD -20
    NEBNext Index 5 Primer for SOLiD -20
    NEBNext Index 6 Primer for SOLiD -20
    NEBNext Index 7 Primer for SOLiD -20
    NEBNext Index 8 Primer for SOLiD -20
    NEBNext Index 9 Primer for SOLiD -20
    NEBNext Index 10 Primer for SOLiD -20
    NEBNext Index 11 Primer for SOLiD -20
    NEBNext Index 12 Primer for SOLiD -20
    NEBNext Index 13 Primer for SOLiD -20
    NEBNext Index 14 Primer for SOLiD -20
    NEBNext Index 15 Primer for SOLiD -20
    NEBNext Index 16 Primer for SOLiD -20
    Product Categories:
    Discontinued
    Applications:
    RNA-seq
    • Properties and Usage
    • Notes
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  • Protocols & Manuals
  • Quality & Safety
    • Quality Control Assays
    • Safety Data Sheets
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