Competent Cells
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  • Competent Cells

    Cloning Strains

    For cloning experiments choose from several high efficiency competent cell strains. These E. coli strains are T1 phage resistant and are endA deficient for high quality plasmid preparations. Additionally, all competent cells from NEB are free of animal products.

    Yeast

    NEB offers chemically competent Kluyveromyces lactis cells and variants of this strain that have been tailored for specific protein expression needs. These cells are suitable for transformation with any of our linearized pKLAC series expression vectors.

    E. coli for Protein Expression

    NEB offers several strains with varying levels of expression control, each with T1 phage resistance and extremely high transformation efficiencies.

    Solutions for the Expression of Difficult Proteins

    NEB has a long history in recombinant protein expression and has developed a wide array of solutions for proteins that are difficult to express.

    NEB has a long history in recombinant protein expression and has developed a wide array of solutions for proteins that are difficult to express.

    Featured Products

    Competent Cells includes these subcategories:

    Cloning Strains Information
    E. coli Expression Strains Information
    Yeast Expression Strains Information

      Publications related to Competent Cells:

    1. Wagner, S., Klepsch, M., Schlegel, S., Appel, A., Draheim, R., Tarry, M., Hö, M., van Wijk, K.J., Slotboom, D.J., Persson, J.O. and de Gier, J. (2008) Tuning Escherichia coli for membrane protein overexpression PNAS 105, 14371-14376.
    2. Narayanan, A., Ridilla, M. and Yernool, D.A. (2010) Restrained expression, a method to overproduce toxic membrane proteins by exploiting operator–repressor interactions Pro. Sci. 20, 51-61.
    3. Schlegel, S., Klepsch, M., Gialama, D., Wickström, D., Slotboom, D.J. and de Gier, J. (2010) Revolutionizing membrane protein overexpression in bacteria  Micro. Biotech. 3, 403-411.

    NEB Competent E. coli Strain Selection

    NEB offers several stains with varying levels of expression control, each with phage T1 resistance and extremely high transformation efficiencies.