Competent Cells

Competent Cells

Cloning Strains

For cloning experiments choose from several high efficiency competent cell strains. These E. coli strains are T1 phage resistant and are endA deficient for high quality plasmid preparations. Additionally, all competent cells from NEB are free of animal products.


NEB offers chemically competent Kluyveromyces lactis cells and variants of this strain that have been tailored for specific protein expression needs. These cells are suitable for transformation with any of our linearized pKLAC series expression vectors.

E. coli for Protein Expression

NEB offers several strains with varying levels of expression control, each with T1 phage resistance and extremely high transformation efficiencies.

  1. How to Perform a Transformation with NEB Competent Cells

    Use this protocol for maximum transformation efficiency.

  2. Tips for Successful Transformations with NEB Competent Cells

    Follow these tips to get superior results.

  3. Solutions for the Expression of Difficult Proteins

    NEB has a long history in recombinant protein expression and has developed a wide array of solutions for proteins that are difficult to express.

Featured Products

Competent Cells includes these subcategories:

Cloning Strains Information
E. coli Expression Strains Information
Yeast Expression Strains Information

    Publications related to Competent Cells:

  1. Narayanan, A., Ridilla, M. and Yernool, D.A. (2010). Restrained expression, a method to overproduce toxic membrane proteins by exploiting operator–repressor interactions Pro. Sci. . 20, 51-61.
  2. Schlegel, S., Klepsch, M., Gialama, D., Wickström, D., Slotboom, D.J. and de Gier, J. (2010). Revolutionizing membrane protein overexpression in bacteria  Micro. Biotech. . 3, 403-411.
  3. Wagner, S., Klepsch, M., Schlegel, S., Appel, A., Draheim, R., Tarry, M., Hö, M., van Wijk, K.J., Slotboom, D.J., Persson, J.O. and de Gier, J. (2008). Tuning Escherichia coli for membrane protein overexpression PNAS . 105, 14371-14376.

NEB Competent E. coli Strain Selection

NEB offers several stains with varying levels of expression control, each with phage T1 resistance and extremely high transformation efficiencies.