ThermoPol® Reaction Buffer Pack

Description

10X ThermoPol Reaction Buffer is optimized for use with VentR® and Deep VentR™ DNA Polymerases. This buffer also provides superior reaction conditions for other thermophilic DNA polymerases, including Taq DNA Polymerase, as well as various other DNA and RNA modifying enzymes. Supplemental MgSO4 is provided to maximize flexibility for users that require additional Mg2+ for their specific application. See individual product descriptions for the recommended buffer for each enzyme, or visit the FAQ page for this product on the NEB web site for a complete list of enzymes for which this buffer is either recommended or compatible.

Reagents Supplied

The following reagents are supplied with this product:

Store at (°C)Concentration
Magnesium Sulfate (MgSO4) Solution-20100 mM

Properties and Usage

Storage Temperature

-20°C

1X Buffer Components

20mM Tris-HCl
10mM (NH4)2SO4
10mM KCl
2mM MgSO4
0.1% Triton® X-100
pH 8.8@25°C

FAQs

  1. What is supplied with the ThermoPol Reaction Buffer pack?
  2. What is the composition of the ThermoPol buffer?
  3. Which Polymerases use this reaction buffer?
  4. How should the NEBuffer be used?
  5. The buffer arrived thawed.  Are the buffer and enzyme still active?

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

Selection Charts

Usage Guidelines & Tips

Interactive Tools

Quality Control

Quality Assurance Statement

  • This reaction buffer and supplement are free of detectable nucleases.

Certificate of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality control's for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Safety Data Sheet

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.