• My NEB
  • Print
  • PDF
  • ThermoPol® Reaction Buffer

    Description

    10X ThermoPol Reaction Buffer is optimized for use with VentR® and Deep VentR™ DNA Polymerases. This buffer also provides superior reaction conditions for other thermophilic DNA polymerases, including Taq DNA Polymerase, as well as various other DNA and RNA modifying enzymes. Supplemental MgSO4 is provided to maximize flexibility for users that require additional Mg2+ for their specific application. See individual product descriptions for the recommended buffer for each enzyme, or visit the FAQ page for this product on the NEB web site for a complete list of enzymes for which this buffer is either recommended or compatible.

    Reagents Supplied

    The following reagents are supplied with this product:

    Store at (°C)Concentration
    Magnesium Sulfate (MgSO4) Solution-20100 mM

    Properties and Usage

    Storage Temperature

    -20°C

    1X Buffer Components

    20mM Tris-HCl
    10mM (NH4)2SO4
    10mM KCl
    2mM MgSO4
    0.1% Triton® X-100
    pH 8.8@25°C

    FAQs

    1. What is supplied with the ThermoPol Reaction Buffer pack?
    2. What is the composition of the ThermoPol buffer?
    3. Which Polymerases use this reaction buffer?
    4. How should the NEBuffer be used?
    5. The buffer arrived thawed.  Are the buffer and enzyme still active?

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

    Quality Control

    Quality Assurance Statement

    • This reaction buffer and supplement are free of detectable nucleases.

    Safety Data Sheet

    The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.