• My NEB
  • Print
  • PDF
  • BSA, Molecular Biology Grade

    Description

    Bovine Serum Albumin (BSA) is supplied with some products to prevent adhesion of the enzyme to reaction tubes and pipette surfaces. BSA also stabilizes some proteins during incubation.

    Properties and Usage

    Storage Temperature

    -20°C

    Storage Conditions

    20 mM Tris-HCl
    100 mM KCl
    0.1 mM EDTA
    50% Glycerol
    pH 8.0 @ 25°C

    Quality Control

    Quality Control Assays

    The following Quality Control Tests are performed on each new lot and meet the specifications designated for the product. Individual lot data can be found on the Product Summary Sheet/Datacard or Manual which can be found in the Supporting Documents section of this page.
    • Endonuclease Activity (Nicking):
      The product is tested in a reaction containing a supercoiled DNA substrate. After incubation for 4 hours the percent converted to the nicked form is determined by agarose gel electrophoresis.
    • Exonuclease Activity (Radioactivity Release):
      The product is tested in a reaction containing a radiolabeled mixture of single and double-stranded DNA. After incubation for 4 hours the exonuclease activity is determined by the % release of radioactive nucleotides.
    • Non-Specific DNase Activity (16 hour):
      The product is tested for non-specific nuclease degradation in a reaction containing a DNA substrate. After incubation for 16 hours there is no detectable degradation of the DNA substrate as determined by agarose gel electrophoresis.
    • Phosphatase activity (FAM labeled oligo):
      The product is tested in a reaction containing a fluorescent internal labeled single stranded oligonucleotide with a 5' phosphate. The percent removal of the 5' phosphate is determined by capillary electrophoresis.
    • Protein Concentration (A280):
      The protein concentration is determined by A280 spectroscopy using the Pace method (Pace, C.N. et al. (1995) Protein Sci., 4, 2411–2423).
    • qPCR DNA Contamination (E. Coli Genomic):
      The product is screened for the presence of E. coli genomic DNA using SYBR® Green qPCR with primers specific for the E.coli 16S rRNA locus. Results are quantified using a standard curve generated from purified E. coli genomic DNA.  The measured level of E. coli genomic DNA contamination is less than 1 E.coli genome.
    • RNase Activity (1 Hour Digestion):
      The product is tested in a reaction containing a RNA substrate.  After incubation for 1 hour there is no detectable degradation of the RNA substrate as determined by gel electrophoresis.
    • RNase Activity (Extended Digestion):
      The product is tested in a reaction containing a RNA substrate. After incubation for 16 hours greater than 90% of the substrate RNA remains intact as determined by gel electrophoresis.
    • Single Stranded DNase Activity (FAM Labeled Oligo):
      The product is tested in a reaction containing a fluorescent internal labeled single stranded oligonucleotide. The percent degradation is determined by capillary electrophoresis.

    References

    1. Pace, C.N. et al. (1995). Protein Sci,. 4, 2411-2423.

    Supporting Documents

    Material Safety Datasheets

    The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.
    1. What is the source of your BSA?
    2. What is the buffer composition of your BSA?
    3. Why do I need to add BSA to my reaction?
    4. Is the BSA in its native form?
    5. Can I use your BSA as a molecular weight marker?
    6. Can I use your BSA as a concentration standard?
    7. Is there a difference between BSA, Molecular Biology Grade and the acetylated BSA you sold previously?
    8. What is the difference between BSA (B9001S) which was discontinued, versus BSA, Molecular Biology Grade (B9000S)?