NEBNext® dA-Tailing Module


The NEBNext® dA-Tailing Module has been optimized to efficiently incorporate a non-templated dAMP on the 3´ end of a blunt DNA fragment (1). 3´-dA DNA tailing prevents concatamer formation during subsequent ligation steps. DNA tailed with the NEBNext dA-Tailing module may be ligated to adaptors or cloning vectors with complementary dT overhangs. The NEBNext dA-Tailing Module is provided as a master mix to maximize efficiency and convenience in DNA sample preparation workflows.

The NEBNext dA-Tailing Module has been validated by sequencing with the an Illumina® Sequencer in conjunction with the NEBNext End Repair Module, NEBNext Quick Ligation Module and Phusion® High-Fidelity PCR Master Mix, and NEBNext High-Fidelity 2X PCR Master Mix.

Reagents Supplied

The following reagents are supplied with this product:

Store at (°C)Concentration
Klenow Fragment (3´→ 5´ exo)-20
NEBNext dA-Tailing Reaction Buffer-2010X

Advantages and Features


  • Efficient – Converts 1-5 μg blunt DNA to DNA with 3´-dAMP overhangs
  • Convenient Reactions are provided in master mix format to reduce steps during DNA sample prep workflows
  • Automation Friendly


  • DNA sample preparation
  • dA-Tailing of 1-5 μg fragmented DNA

Properties and Usage

Storage Temperature



  1. Clark, J.M. et al. (1987). J. Mol. Biol.. 198(1), 123-127.
  2. Derbyshire, V. et al. (1988). Science. 240, 199-201.


  1. What type of sample prep/library construction can I use the dA-Tailing Module reagents for?
  2. How much DNA can I dA-Tail in one NEBNext dA-Tailing Reaction?


  1. NEBNext dA-Tailing Module Protocol (E6053)


The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name these document files: manual[Catalog Number].

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Safety Data Sheet

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.