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  • NEBNext® dA-Tailing Module

    Description

    The NEBNext® dA-Tailing Module has been optimized to efficiently incorporate a non-templated dAMP on the 3´ end of a blunt DNA fragment (1). 3´-dA DNA tailing prevents concatamer formation during subsequent ligation steps. DNA tailed with the NEBNext dA-Tailing module may be ligated to adaptors or cloning vectors with complementary dT overhangs. The NEBNext dA-Tailing Module is provided as a master mix to maximize efficiency and convenience in DNA sample preparation workflows.

    The NEBNext dA-Tailing Module has been validated by sequencing with the an Illumina® Sequencer in conjunction with the NEBNext End Repair Module, NEBNext Quick Ligation Module and Phusion® High-Fidelity PCR Master Mix, and NEBNext High-Fidelity 2X PCR Master Mix.


    Reagents Supplied

    The following reagents are supplied with this product:

    Store at (°C)Concentration
    Klenow Fragment (3´→ 5´ exo)-20
    NEBNext dA-Tailing Reaction Buffer-2010X

    Advantages and Features

    Features


    • Efficient – Converts 1-5 μg blunt DNA to DNA with 3´-dAMP overhangs
    • Convenient Reactions are provided in master mix format to reduce steps during DNA sample prep workflows
    • Automation Friendly

    Applications


    • DNA sample preparation
    • dA-Tailing of 1-5 μg fragmented DNA

    Properties and Usage

    Storage Temperature

    -20°C

    Notes

    1. For larger volume requirements, customized and bulk packaging is available by purchasing through the OEM/Bulks department at NEB. Please contact OEM@neb.comfor further information.

    References

    1. Clark, J.M. et al. (1987). J. Mol. Biol.. 198(1), 123-127.
    2. Derbyshire, V. et al. (1988). Science. 240, 199-201.

    Supporting Documents

    Material Safety Datasheets

    The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.

    Manuals

    The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name these document files: manual[Catalog Number].

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.
    1. What type of sample prep/library construction can I use the dA-Tailing Module reagents for?
    2. How much DNA can I dA-Tail in one NEBNext dA-Tailing Reaction?
    1. NEBNext dA-Tailing Module Protocol (E6053)