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  • Diluent C


    Diluent C is recommended for making dilutions of New England Biolabs, Inc. restriction enzymes.

    When necessary, we recommend diluting enzymes just prior to use and suggest that the final concentration of diluted enzymes be at least 1,000 units/ml. Enzymes should be stored at their selling concentrations.

    Each vial contains 1.3 ml of diluent buffer.

    Properties and Usage

    Storage Temperature


    1X Buffer Components

    250mM NaCl
    10mM Tris-HCl
    1mM DTT
    0.1mM EDTA
    0.15% Triton® X-100
    200μg/ml BSA
    50% Glycerol
    pH 7.4@25°C


    1. These enzyme diluent buffers are not for use with VentR® DNA Polymerases. For diluting VentR® DNA Polymerases, use (NEB #B8004S).

    Supporting Documents

    Material Safety Datasheets

    The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.


    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.
    1. What is supplied with the Diluent Buffer C?
    2. What is the composition of the Diluent Buffer C?
    3. How should the Diluent Buffer be used?
    4. How long can the diluted enzyme be stored?