< Return to "NEBNext® Ultra™ II RNA Library Prep (non-directional): Increasing Library Yields"
 
 
 

NEBNext Ultra II RNA produces yields several fold higher than the original Ultra RNA Kit
Nondir_RNA_Yield_Figure3
Poly(A)-containing mRNA was isolated from Human Universal Reference RNA (Agilent #740000) using the NEBNext Poly(A) mRNA Magnetic Isolation Module, and libraries were made using either the Ultra II RNA Kit or the original Ultra RNA Kit. Significantly higher yields were achieved with the Ultra II RNA kit than with the original Ultra RNA Kit.

One or more of these products are covered by patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc. For more information, please email us at [email protected]. The use of these products may require you to obtain additional third party intellectual property rights for certain applications.

ILLUMINA®, TRUSEQ® and NEXTSEQ® are registered trademarks of Illumina, Inc.
SPRISELECT® is a registered trademark of Beckman Coulter, Inc.
RIBO-ZERO™ is a trademark of Illumina, Inc.
KAPA™ is a trademark of Kapa Biosystems

Available Kits:

NEBNext_portal_product_icon 

NEBNext® Ultra™ II RNA Library Prep Kit for Illumina®
Includes optimized mixes for non-directional RNA library preparation (fragmentation, cDNA synthesis, end repair/dA-tailing, adaptor ligation and PCR enrichment steps) for sequencing on the Illumina platform.

NEBNext_portal_product_icon 

NEBNext® Ultra™ II RNA Library Prep with Sample Purification Beads
Includes optimized mixes for non-directional RNA library preparation (fragmentation, cDNA synthesis, end repair/dA-tailing, adaptor ligation and PCR enrichment steps) plus SPRIselect® beads for size selection and cleanup.