NEBinspired is a science blog designed to share inspirational stories about trends in the life sciences, lab tips to help you save time, and life lessons to reflect on. Browse our collection of science stories, or filter to find a topic that you are passionate about. From groundbreaking discoveries to sustainable lab techniques and helpful online tools that will aid your experimental design, we have something for everyone. And if you can’t find what you’re looking for, not to worry – simply send us a message about topics you would like to see covered.

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Bisulfite sequencing, but at what cost?

Posted on Thursday, February 17, 2022 By Nicole Kelesoglu

Why do so many core sequencing labs decline to perform bisulfite-seq? Let’s review the intrinsic risks of bisulfite conversion and then discuss how enzymatic cytosine conversion overcomes these challenges, enabling more biologically meaningful results at a lower cost with application versatility.

Topic: Tips for the lab

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Bringing more COVID-19 testing to Africa

Posted on Thursday, January 27, 2022 By

Lydia Morrison, M.S.

& Tessa DeVoe

With so few Africans vaccinated against COVID-19, simple and sensitive tests are critical for saving lives.  NEB is donating reagents to aid in the development of diagnostic tests (cLAMP) that can be used instead of PCR and can deliver rapid results detected by a simple color change.  They are ideally suited for resource-poor areas by not requiring expensive laboratory equipment.

Topic: Environment and social responsibility

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COVID testing In Africa Blog

Proven tips for PCR primer design

Posted on Monday, November 8, 2021 By Nicole Kelesoglu

PCR optimization and validation is a longtime passion at NEB. Avoid common PCR pitfalls and obtain the amplicon you desire with these primer design tips from our experts.

Topic: Tips for the lab

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DNA molecules and nucleotide bases

Using large-scale RNA sequencing to probe immune response, including autoimmune disease and vaccination

Posted on Wednesday, October 13, 2021 By Joanne Gibson, Ph.D.

Researchers are efficiently exploring immune responses with a new bulk RNA sequencing workflow that generates full-length reads of B or T cell receptor V(D)J and (C) region mRNA. The discoveries incorporating this unique view are shedding light on previously unanswerable questions surrounding immune response to infection and therapeutic intervention.

Topic: What is Trending in Science

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antibody and cell

8 tips to follow when choosing a restriction enzyme for in vitro mRNA vaccine production

Posted on Friday, September 24, 2021 By Penny Fu Devoe, Associate Director, Portfolio Management, DNA Cloning & Joanne Gibson, Ph.D.

mRNA vaccines have emerged as alternatives to conventional vaccine approaches because of the streamlined path from early discovery and development to a point-of-care therapeutic. In this post, read practical tips to consider when choosing a restriction enzyme for one of the crucial first steps of IVT vaccine development - linearizing the plasmid that contains the vaccine target sequence.

Topic: What is Trending in Science

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Restriction enzymes for use in mRNA vaccine development

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