Customers often ask us how they can maximize yield when using our Monarch Miniprep Kit. Here are a few helpful tips.
Resuspend your pellet completely. Any bacteria that haven't been resuspended won't lyse efficiently, and the amount of DNA that you will obtain will be lower.
When lysing the cells, invert the tubes several times so that the solution becomes viscous and uniformly darker pink.
Make sure that you add the elution buffer to the center of the matrix without puncturing it. The elution buffer needs to be in contact with the column matrix for efficient elution. Be sure to incubate the elution buffer in the column for a full minute before spinning.
Remember, if you have any additional questions, you can always contact our tech support team at info@neb.com.
Help us celebrate our 50th anniversary! We have hidden 1,000 golden butterflies and are waiting for you to find them. They can be anywhere that you find NEB! Beginning April 15th, be sure to visit our website and tables at tradeshows and events you are attending. Visit our social media channels frequently for tips on where we have hidden the butterflies – and once you find one, either click or scan the code to be eligible for a 50th anniversary prize pack, as well as a grand prize trip to NEB headquarters in Ipswich, MA!.
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