NEB Scientific Posters
Your search returned 101 results.
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NEBNext enzymatic solutions for challenging samples & workflows
Improvements in library quality and variant calling accuracy of NEBNext UltraShear® and NEBNext UltraShear FFPE DNA Library Prep to FFPE Samples in a hybrid capture workflow.
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NEBNext UltraShear® Long Read: Novel and time-dependent enzymatic DNA fragmentation for long read sequencing
Novel solution for enzymatic fragmentation for long-read sequencing.
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Optimized NEBNext UltraExpress® workflows enable high-performance RNA and DNA library construction from low inputs
Application and performance recommendations of NEBNext UltraExpress DNA and RNA for low-input samples.
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Accurate representation of small RNAs using NEBNext Low-bias Small RNA Library Prep Kit
Novel single-day ligation-based small RNA library preparation method demonstrates reduced bias, enhanced sensitivity, and accuracy of sncRNA detection.
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Enzymatic Methyl-seq enables simultaneous analysis of methylation and germline variants from whole genome and target capture data
NEBNext® Enzymatic Methyl-seq generates consistent and accurate methylation calls using various sample types across a wide range of inputs. The use of base conversion in EM-seq™ poses a challenge to variant detection, however, this can be resolved bioinformatically because methylation information is preserved on only one strand in EM-seq libraries, while the other strand can be used to detect genetic variation. Here we present a bioinformatic workflow that demonstrates the ability to call germline SNPs with high recall and precision. With this ability to assess methylation state and genetic mutations from a single library we can maximize the utility of our sequencing datasets.
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NEBNext® enzymatic solutions for DNA methylation profiling of highly damaged DNA inputs
Fragmented or low-quality DNA, including FFPE DNA, can be challenging to analyze for epigenetic marks, but new techniques demonstrate reliably high-quality methyl-sequencing profiles. Enzymatic fragmentation, when used upstream of enzymatic methylation mapping, improves library data quality metrics as compared to mechanical shearing with Covaris.
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A Sample-to-results Workflow for Agnostic or Targeted Pathogen Sequencing from Wastewater
Population-level pathogen surveillance of wastewater can identify disease outbreaks early. In this poster, we present a workflow for detecting the example pathogens, influenza A (flu A) and respiratory syncytial virus (RSV), with NEBNext library prep in concert with Nanotrap Microbiome A and B particles.
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New single day EM-seq and E5hmC-seq workflows: Faster library prep for whole genome and target enrichment methylome profiling
In this poster, we demonstrate a faster, single-day workflow for NEBNext Enzymatic Methyl-seq v2 and NEBNext Enzymatic 5hmC-seq, featuring a reduced deamination time.
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Cell free DNA detection workflows utilizing the Monarch Mag Cell free DNA cfDNA Extraction Kit
This poster demonstrates the successful use of Monarch Mag Cell-free DNA for cfDNA extraction from liquid biopsy samples, leading to high-quality DNA library prep using a modified NEBNext Ultra II DNA library extraction protocol.
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High-Throughput NEBNext Library Preparation Solutions Enabled
Automation of NGS library prep, enabled by robotic liquid handlers, meets the needs of NGS across a variety of utilities including basic research, drug discovery, and human Health. New England Biolabs and NEBNext® NGS solutions are designed with automation in mind by addressing several factors including fewer workflow components, streamlined workflows, reduction in bead cleanups, novel enzymatic solutions, and generous overages to support automation dead-volumes.
