NEBuilder® HiFi DNA Assembly

NEBuilder elephants

Choose NEBuilder HiFi

NEBuilder HiFi DNA Assembly enables virtually error-free joining of DNA fragments, even those with 5´- and 3´-end mismatches. This flexible mix enables simple and fast seamless cloning utilizing a proprietary high-fidelity polymerase. Find out why NEBuilder HiFi is the next generation of DNA assembly and cloning.

NEBBuilder Simplicity

Enjoy simple and fast seamless cloning in as little as 15 minutes

NEBuilder® Assembly Tool

NEBuilder Assembly Tool can be used to design primers for your NEBuilder HiFi DNA Assembly or Gibson Assembly® reactions.

Try Our Tool
Bridging dsDNA with a ssDNA oligo and NEBuilder HiFi to create an sgRNA-Cas9 Expression Vector

NEB has developed a protocol for construction of an sgRNA or sgRNA library into a Cas9/sgRNA expression vector using single-stranded DNA oligonucleotides, a restriction enzyme digested vector and the NEBuilder HiFi DNA Assembly Master Mix.

View Application Note
Improved methods for site-directed mutagenesis using NEBuilder HiFi DNA Assembly Master Mix

Here we describe the use of the NEBuilder HiFi DNA Assembly Master Mix to generate multiple site-directed mutagenesis at the same time.

View Application Note

Featured NEBuilder Products

NEBuilder HiFi DNA Assembly mix is available alone or bundled with competent cells.  Please inquire for large volume purchases.

This content is covered by patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc. For more information, please email us at The use of these products may require you to obtain additional third party intellectual property rights for certain applications.

Loading Spinner