FAQ: What fragment sizes can be generated with TnX® Transposase (Unloaded) (NEB #M2623)?

Fragment size is influenced by DNA input amount, transposome concentration, and reaction conditions. Under standard unit assay conditions, loaded TnX transposome generates average fragment sizes of approximately 500-600 bp. Users can adjust reaction parameters, specifically DNA input to transposome ratio, to tailor fragment size distributions for specific downstream applications.