Yes. NEBNext UltraShear Long Read is designed for automation, with minimal sample transfers, sample tracking flexibility, and sufficient reagent overages to account for automation dead volumes.
When processing High Molecular Weight gDNA, automation instruments may cause additional shearing compared to manually processed NEBNext UltraShear Long Read samples. It is therefore recommended to decrease the incubation time during sample fragmentation on automated platforms relative to manual preparations. Incubation times should be optimized based on the specific sample type and instrument being used.
For optimal performance in an automation workflow, NEBNext® UltraShear Long Read Master Mix should be pipette-mixed immediately before distribution to samples. During mixing and subsequent addition to samples, gently aspirate and dispense from the bottom of the wells and limit blowout steps. This will minimize bubble formation, ensure precise reagent aspiration, and promote proper master mix–sample mixing.
