HomeFAQsHow important is the volume of isopropanol in the NEBNext Low-bias Small RNA Library Prep Kit (NEB #E3420) protocol?
FAQ: How important is the volume of isopropanol in the NEBNext Low-bias Small RNA Library Prep Kit (NEB #E3420) protocol?
The proper addition of isopropanol during Section 6 of the Product Manual (Size Selection) is essential for the binding of the small RNA library cDNA to the NEBNext Sample Purification Beads. We recommend pre-wetting of pipette tips for the addition of isopropanol.
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