FAQ: Does E.coli DNA Gyrase work in other NEBuffers?
When NEBuffers are supplemented with 1.75 mM ATP final concentration, the observed activity is:
NEBuffer™ r1.1: 0%
NEBuffer™ r2.1: 0%
NEBuffer™ r3.1: 0%
rCutSmart™ Buffer: 12.5%
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