Home FAQs Does 9°N™ Reverse Gyrase (NEB #M0200) work in other NEBuffers?

FAQ: Does 9°N™ Reverse Gyrase (NEB #M0200) work in other NEBuffers?

9°N™ Reverse Gyrase works best in the supplied buffer. When the NEBuffers are supplemented with 1.75 mM ATP final concentration, the observed activity is:

  • NEBuffer 1: ~25%
  • NEBuffer 2: ~50%
  • NEBuffer r2.1: ~75%
  • NEBuffer 3: ~50%
  • NEBuffer r3.1: ~50%
  • NEBuffer 4: ~50%
  • rCutSmart Buffer: ~50%