FAQ: Do the magnetic beads in the Monarch® Mag PCR & DNA Cleanup Kit (5 μg) (NEB #T4130) need to be completely dry before eluting DNA?

Proper drying of magnetic beads is a critical step to ensure optimal DNA recovery and purity. Beads should be dried just enough to eliminate any visible free-flowing liquid, while still retaining a shiny, dark brown appearance. This indicates they are ready for elution.

Avoid over-drying, as it can negatively impact DNA recovery. Over-dried beads typically appear matte and light brown and may be more difficult to resuspend.