RNA Capping Enzyme Selection Chart by Application
Please click here for capping enzyme properties.
| NEB Product |
Catalog # | Applications | Incubation activity range (preferred) | Tag |
|---|---|---|---|---|
| RNA Capping Enzymes | ||||
| Faustovirus Capping Enzyme (FCE) | M2081 | Post-transcriptional Cap-0 RNA synthesis. Post-transcriptional , one-pot Cap-1 RNA synthesis with mRNA Cap 2´-O-Methyltransferase. Post-transcriptional, one-pot Cap-1 m6A RNA synthesis with mRNA Cap 2´-O-Methyltransferase and mRNA Cap N6 Methyltransferase (m6A). |
37-50°C | His |
| Vaccinia Capping System (VCE) | M2080 | Post-transcriptional Cap-0 RNA synthesis. Post-transcriptional, one-pot Cap1-RNA synthesis with mRNA Cap 2´-O-Methyltransferase. Post-transcriptional, one-pot Cap-1 m6A RNA synthesis with mRNA Cap 2´-O-Methyltransferase and mRNA Cap N6 Methyltransferase (m6A). Cappable Seq. ReCappable Seq. |
37-50°C | His |
| 5´ Cap Modifications | ||||
| mRNA Cap 2´-O-Methyltransferase | M0366 | In vivo mRNA use. One-step capping with FCE/VCE. | 25-37°C (37°C) | His |
| mRNA Cap N6 Methyltransferase (m6A) | M0767 | Conversion of Cap-1 with initiating +1 A to m6A-Cap-1. Post-transcriptional Cap-1 m6A RNA synthesis with FCE or VCE and mRNA Cap 2´-O-methyltransferase. | 25-40°C (37°C) | His |
