FAQ: How does NEBridge Ligase Master Mix perform in Golden Gate Assembly? How does it compare to “home brew” protocols?

Golden Gate Assembly using NEBridge Ligase Master Mix with seven NEB Type IIS restriction enzymes has been optimized to achieve high efficiency and fidelity, even with complex assemblies of 25 fragments. Depending on the complexity of your assembly reaction and your choice of Type IIS restriction enzyme, you can expect 106-108 cfu per µg vector DNA with a correct assembly rate exceeding 80%. We recognize that many customers prefer to home brew and do this themselves. However, our hope is that by choosing this reagent, we can remove a technical obstacle from your workflow and help you achieve more successful assemblies.