FAQ: How do I choose primers for cDNA synthesis using LunaScript RT Master Mix (Primer-free)?

The optimal primer for cDNA synthesis depends on the desired downstream application.

Downstream Applications Primers Goal
qPCR quantitation Random Primer Mix (NEB #S1330S)
Typical recommendation: 6 µM final
Even coverage
General cDNA synthesis Oligo d(T)23VN (NEB #S1327S)
Typical recommendation: 5 µM final
Full-length cDNA synthesis
Random Primer Mix or Random Primer
Typical recommendation: 6 µM final
Even coverage
Gene-specific Primer
Typical recommendation: 5 µM final
(Range: 0.1 - 1 µM)
Target-specific cDNA