FAQ: What should the final loading concentration be for MiSeq® and NextSeq® 500/550 Illumina® sequencing platforms?

The final loading concentration depends on the Illumina instrument and sequencing reagents. For MiSeq sequencing with V2 or V3 reagents, we recommend 8-10 pM. For NextSeq 500, we recommend 1.2-1.4 pM.