HomeFAQsWhat sequencing depth is required for immune sequencing libraries?
FAQ: What sequencing depth is required for immune sequencing libraries?
The sequencing depth requirement is highly dependent on the biological questions that one is trying to answer. Typically, 500,000 reads per library is a good starting point for most projects to saturate a detection of 3000-4000 clonotypes. If the immune repertoire diversity of the RNA sample is higher, more sequencing reads are needed to detect all the low frequency clonotypes.