HomeFAQsThe Type IIS restriction enzyme used in this kit is BsmBI-v2. How does it compare to the original BsmBI or Esp3I?
FAQ: The Type IIS restriction enzyme used in this kit is BsmBI-v2. How does it compare to the original BsmBI or Esp3I?
While all 3 can perform complex (24-fragment) assemblies, BsmBI-v2 had the highest efficiency (number of transformants) and fidelity (% correctly assembled constructs) levels of the three. These comparison experiments used the individually optimized temperatures for each enzyme, 42°C for BsmBI and BsmBI-v2, and 37°C for Esp3I.
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